Herrmann P, Schreier M H, Bazin H, Zinkernagel R M, Cerny A
Department of Preclinical Research, Pharmaceutical Division Sandoz Ltd., Basel, Switzerland.
Immunobiology. 1988 Sep;177(4-5):382-9. doi: 10.1016/S0171-2985(88)80006-8.
The induction and the effector phase of murine delayed type hypersensitivity (DTH) were evaluated in mice treated from birth with anti-IgM antibodies; these mice had no mature B cells and could not produce an antibody response. To study the effector phase, long-term cultured cloned helper T cells were injected subcutaneously together with the specific antigen into the hind footpad of normal and B cell-deficient mice. Antigen-specific DTH responses assessed by the local swelling reaction 24 h after transfer measured against a particulate antigen (sheep red blood cells, SRBC) as well as a soluble antigen (ovalbumin, OVA) were unaffected by the absence of B cells. To study the induction phase of DTH, 3-day immune in vivo primed lymphocytes from normal or B cell-depleted mice were adoptively transferred by subcutaneous injection into the hind footpad of naive syngeneic recipients. B cell depletion did not affect the induction of cells capable of responding to SRBC; in contrast, the response to soluble antigen (OVA) was significantly reduced, suggesting that B cells or their products participated in the induction of a DTH response to a soluble antigen.
在从出生就用抗IgM抗体处理的小鼠中评估了小鼠迟发型超敏反应（DTH）的诱导期和效应期；这些小鼠没有成熟的B细胞，不能产生抗体反应。为了研究效应期，将长期培养的克隆辅助性T细胞与特异性抗原一起皮下注射到正常和B细胞缺陷小鼠的后足垫中。通过在转移后24小时针对颗粒性抗原（绵羊红细胞，SRBC）以及可溶性抗原（卵清蛋白，OVA）测量的局部肿胀反应评估的抗原特异性DTH反应不受B细胞缺失的影响。为了研究DTH的诱导期，将来自正常或B细胞耗竭小鼠的3天体内免疫致敏淋巴细胞通过皮下注射过继转移到同基因的未致敏受体的后足垫中。B细胞耗竭不影响对SRBC有反应的细胞的诱导；相反，对可溶性抗原（OVA）的反应显著降低，表明B细胞或其产物参与了对可溶性抗原的DTH反应的诱导。
