State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.
J Plant Res. 2020 Sep;133(5):727-737. doi: 10.1007/s10265-020-01213-z. Epub 2020 Jul 8.
NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both Nicotiana tabacum and Arabidopsis thaliana. Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 protein was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that in the transgenic tobacco the salt tolerance was enhanced, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress.
NACs 是植物中主要的转录因子家族之一，在植物生长发育以及逆境胁迫响应中发挥重要作用。本研究从杨树品种 84K 中克隆了一个盐诱导的 NAC 转录因子基因（NAC13），并将其转化到烟草和拟南芥中。35S::NAC13-GFP 融合蛋白在拟南芥中的稳定表达分析表明，NAC13 蛋白定位于细胞核。我们还获得了五个转基因烟草株系。形态学和生理学特征以及盐处理分析的证据表明，在转基因烟草中，盐耐受性增强，表明 NAC13 基因可能作为烟草响应盐胁迫的正调控因子发挥作用。
