State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Co-Innovation Center for Sustainable Forestry in Southern China/Bamboo Research Institute, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2021 Mar 6;22(5):2656. doi: 10.3390/ijms22052656.
Plant growth and development are challenged by biotic and abiotic stresses including salinity and heat stresses. For as an important greening and economic tree species in China, increasing soil salinization and global warming have become major environmental challenges. We aim to unravel the molecular mechanisms underlying tree tolerance to salt stress and high temprerature (HT) stress conditions. Transcriptomics revealed that a transcription factor (TF) was significantly induced by salt stress in . This study focuses on addressing the biological functions of . The gene was cloned, and its temporal and spatial expression was analyzed under different stresses. was significantly upregulated under 150 mM NaCl and 37 °C for 12 h. The result is consistent with the presence of stress responsive -elements in the promoter. Subcellular localization analysis showed that was targeted to the nucleus. Additionally, was highly expressed in the leaves and roots. To investigate the core activation region of protein and its potential regulatory factors and targets, we conducted trans-activation analysis and the result indicates that the C-terminal region of 191-343 amino acids of the was a potent activation domain. Furthermore, overexpression of stimulated plant growth and enhanced salinity and HT tolerance. Moreover, 14 stress-related genes upregulated in the transgenic plants under high salt and HT conditions may be potential targets of the . All the results demonstrate that plays an important role in salt and HT stress tolerance.
植物的生长和发育受到生物和非生物胁迫的挑战，包括盐度和热胁迫。因为作为中国重要的绿化和经济树种，土壤盐渍化和全球变暖已成为主要的环境挑战。我们旨在揭示树木耐受盐胁迫和高温（HT）胁迫条件的分子机制。转录组学表明，在中，一个转录因子（TF）被盐胁迫显著诱导。本研究重点研究的生物学功能。克隆了该基因，并分析了其在不同胁迫下的时空表达。在 150 mM NaCl 和 37°C 下处理 12 小时后，表达量显著上调。这一结果与 启动子中存在应激响应元件一致。亚细胞定位分析表明，定位于细胞核。此外，在叶片和根中高度表达。为了研究 蛋白的核心激活区及其潜在的调控因子和靶标，我们进行了转录激活分析，结果表明 191-343 个氨基酸的 C 末端区域是一个有效的激活结构域。此外，过表达 可促进植物生长，增强耐盐性和 HT 胁迫。此外，在高盐和 HT 条件下，转基因植物中 14 个与应激相关的基因上调，可能是 的潜在靶标。所有结果表明，在盐和 HT 胁迫耐受性中发挥重要作用。
