State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China; Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing, 100091, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin, 150040, China.
Plant Sci. 2019 Apr;281:1-8. doi: 10.1016/j.plantsci.2019.01.003. Epub 2019 Jan 7.
Transcription factor (TF) genes play a critical role in plant abiotic and biotic stress responses. In this study, we cloned a poplar TF NAC13 gene (Potri.001G404100.1), which is significantly up-regulated to salt stress. Then we developed gene overexpression and antisense suppression constructions driven by CaMV35S, and successfully transferred them to a poplar variety 84 K (Populus alba × P. glandulosa), respectively. Evidence from molecular assay indicated that NAC13 overexpression and antisense suppression fragments have been integrated into the poplar genome. The morphological and physiological characterization and salt treatment results indicated the NAC13-overexpressing transgenic plants enhance salt tolerance significantly, compared to wide type. In contrast, the NAC13-suppressing transgenic plants are significantly sensitive to salt stress, compared to wide type. Evidence from transgenic Arabidopsis expressing GUS gene indicated that the gene driven by NAC13 promoter is mainly expressed in the roots and leaves of young plants. These studies indicate that the NAC13 gene plays a vital role in salt stress response.
转录因子（TF）基因在植物非生物和生物胁迫反应中起着关键作用。在这项研究中，我们克隆了杨树 TF NAC13 基因（Potri.001G404100.1），该基因在盐胁迫下显著上调。然后，我们分别构建了由 CaMV35S 驱动的基因过表达和反义抑制载体，并成功地将它们转化到一个杨树品种 84K（Populus alba × P. glandulosa）中。分子检测证据表明，NAC13 过表达和反义抑制片段已整合到杨树基因组中。形态和生理特征以及盐处理结果表明，与野生型相比，NAC13 过表达转基因植物显著提高了耐盐性。相比之下，与野生型相比，NAC13 抑制的转基因植物对盐胁迫非常敏感。表达 GUS 基因的转基因拟南芥的证据表明，由 NAC13 启动子驱动的基因主要在幼苗的根和叶中表达。这些研究表明，NAC13 基因在盐胁迫反应中起着至关重要的作用。
