Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
J Med Virol. 2020 Dec;92(12):3815-3820. doi: 10.1002/jmv.26283. Epub 2020 Jul 17.
Distinct human papillomavirus (HPV) 18 variants are thought to differ in oncogenic potential and geographic distribution. As such, understanding the regional variants of HPV 18 would be of great importance for evolutionary, epidemiological, and biological analysis. In this regard, the sequence variations of E6 gene were investigated to characterize more common variants of HPV 18 in normal cells, premalignant, and malignant samples collected from the cervix. In total, 99 samples of HPV 18 were analyzed by polymerase chain reaction and sequencing. In overall, lineages A was identified in all study subjects, among which sublineage A4 was dominant although the difference observed was not statistically significant with regard to different stages of disease. Sublineage A4 comprised 90.9% of samples and the remaining were belonged to sublineages A1, A2, A3, and A5 at the frequency of 6.1%, 1%, 1%, and 1%, respectively. In conclusion, our findings clearly highlight the sublineage A4 of HPV 18 as the most dominant variant in Iran.
人们认为，不同的人乳头瘤病毒（HPV）18 变体在致癌潜力和地理分布上存在差异。因此，了解 HPV 18 的区域变体对于进化、流行病学和生物学分析非常重要。在这方面，我们研究了 E6 基因的序列变异，以描述从宫颈采集的正常细胞、癌前病变和恶性样本中 HPV 18 的常见变体。总共分析了 99 个 HPV 18 样本的聚合酶链反应和测序。总的来说，所有研究对象中都鉴定出线粒体 A，其中亚系 A4 占主导地位，尽管在疾病不同阶段观察到的差异没有统计学意义。亚系 A4 占样本的 90.9%，其余分别属于亚系 A1、A2、A3 和 A5，频率分别为 6.1%、1%、1%和 1%。总之，我们的研究结果清楚地表明，HPV 18 的亚系 A4 是伊朗最主要的变体。
