Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 14155, Iran.
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
Virol J. 2024 Oct 3;21(1):244. doi: 10.1186/s12985-024-02521-9.
Variant analysis of distinct HPV types is important from different aspects including epidemiology, pathogenicity, and evolution.
For this reason, the full sequence of the E6 and E7 genes of HPV 58 was examined in 130 HPV 58-infected cervical samples using PCR and sequencing.
Our results revealed that three lineages A, B, and D were found in this study; among which the B lineage was more common (91.50%). About sublineages, all samples of the B lineage belonged to the B1 sublineage, and samples that were classified as the A and D lineages were found to belong to the A1 (0.77%), A2 (5.38%), A3 (1.50%), and D2 (0.77%) sublineages. No statistically significant differences were found between lineages and stages of disease or amino acid changes (P > 0.05).
Our results showed that lineage B, sublineage B1, was dominant in Iran. However, more studies with larger sample sizes from different parts of Iran are essential for assessing the pathogenicity risk of HPV 58 lineages in Iranian women with cervical cancer.
不同 HPV 类型的变异分析从流行病学、致病性和进化等方面都很重要。
为此，本研究采用 PCR 和测序的方法，对 130 例 HPV 58 感染的宫颈样本中的 HPV 58 的 E6 和 E7 基因全长序列进行了检测。
本研究发现存在 A、B 和 D 三个谱系；其中 B 谱系更为常见（91.50%）。关于亚谱系，B 谱系的所有样本均属于 B1 亚谱系，而属于 A 和 D 谱系的样本则属于 A1（0.77%）、A2（5.38%）、A3（1.50%）和 D2（0.77%）亚谱系。谱系与疾病分期或氨基酸变化之间无统计学差异（P＞0.05）。
本研究结果表明，伊朗以 B 谱系、B1 亚谱系为主。然而，对于伊朗不同地区更大样本量的进一步研究对于评估 HPV 58 谱系在伊朗宫颈癌妇女中的致病性风险至关重要。
