Choi Joo Hee, Kim Namyoung, Rim Min A, Lee Wonchan, Song Jeong Eun, Khang Gilson
Department of BIN Convergence Technology and Department of Polymer Nano Science & Technology Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeollabuk-do, Republic of Korea.
ACS Appl Mater Interfaces. 2020 Aug 5;12(31):34703-34715. doi: 10.1021/acsami.0c10415. Epub 2020 Jul 23.
Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions. However, advancing a proper model for OC TE is still under way. Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model. The BH composite can be fabricated easily with a cell-friendly biomaterial and cross-linker. The BH composite was characterized by a morphological method and physicochemical aspect. The mechanical and rheological characters were further confirmed to verify its applicability in OC TE. The thermodynamic property of the composite was determined to analyze thermal stability and interaction among matrices. The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct. studies were carried out to study the viability and biochemical characters of the developed biomaterial. An study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites. The data displayed stable physicochemical properties and mechanical characters when the DBPs were incorporated with a proper amount. The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation. The cytotoxicity of the fabricated material was low, which allows application and . The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model. The long-term study displayed excellent biocompatibility and great potential in the OC defected region. Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.
骨软骨（OC）组织工程（TE）是一种用于再生急性或退行性软骨及OC损伤的有前景的策略。然而，推进合适的OC TE模型仍在进行中。在此，基于结冷胶（GG）水凝胶和脱矿骨颗粒（DBP）的双层水凝胶（BH）被提议作为一种新模型。BH复合材料可以用对细胞友好的生物材料和交联剂轻松制备。通过形态学方法和物理化学方面对BH复合材料进行了表征。进一步确认了其力学和流变特性，以验证其在OC TE中的适用性。测定了复合材料的热力学性质，以分析热稳定性和基质间的相互作用。通过将材料置于模拟体液（SBF）溶液中处理28天来研究材料的生物活性，以检查BH构建体中晶体结构的形成。开展了研究以研究所开发生物材料的活力和生化特性。进行了一项研究以分析材料的生物相容性以及植入BH复合材料的损伤OC区域的再生情况。数据显示，当以适量加入DBP时，其具有稳定的物理化学性质和力学特性。负载DBP的水凝胶的生物活性显示出大量磷灰石形成。所制备材料的细胞毒性较低，这允许其应用。生化研究显示出高水平的碱性磷酸酶（ALP）活性和基因表达，这表明负载DBP的GG在BH模型的骨层中具有良好的应用前景。长期研究显示出优异的生物相容性以及在OC缺损区域的巨大潜力。总体而言，这些结果表明联合和创新方法对于改善OC再生治疗策略的重要性，并且本研究中提出的BH模型可能是一种有前景的用于OC TE的生物材料模型。
