Department of Bionanotechnology and Bio-Convergence Engineering, Department of Polymer Nano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, Deokjin-gu, Jeonju 561-756, Republic of Korea.
Microsystem Technology Group, Center for Materials and Microsystems, Fondazione Bruno Kessler, via Sommarive 18, Trento 38123, Trentino, Italy.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1900-1911. doi: 10.1021/acsabm.0c01549. Epub 2021 Jan 22.
Bone is the rigid tissue that constitutes the skeleton. The material for bone regeneration has to provide the mechanical stability by maintaining the mechanical loads both in the rest conditions and during the body movements. Bone is dynamic tissue constantly reshaped by the action of cells (osteoblasts and osteoclasts). This activity is normally enough to heal bone injuries; however, in several conditions, when bone is subjected to fatal damages, self-renewal is difficult, if not even impossible, and a medical treatment is required. The transplantation of a biomaterial is one of the common surgical procedures to overcome critical injuries. In this study, we exploited the effect of the use of different sources of demineralized bone powder (DBP) in combination with gellan gum (GG) to form a GG-DBP hydrogel scaffold with the purpose of regenerating the bone tissue. DBP was extracted from the femurs of two typologies of domesticus (the Yeonsan Ogye, a traditional and rare black chicken from Korea, and the Cornish cross, the most common breeds for industrial meat production) and the Pekin duck. The composite scaffold has been tested both and . studies using rat bone marrow-derived mesenchymal stem cells (rBMSCs) confirmed the cellular suitability of bone-specific gene expression for seeded GG-DBP scaffolds, differentiation capacity, and marked upregulation. The scaffold containing a DBP derived from the Yeonsan Ogye (YO) bone showed higher levels of cell proliferation and osteogenic differentiation in comparison with the scaffold with the DBP obtained from the other studied sources. These results have been related with the higher amount of melanin, studied by fluorescence, of the YO DBP compared to Cornish cross and Pekin duck. Overall, this study clearly shows the use of YO DBP as a promising material in bone tissue regeneration.
骨是构成骨骼的刚性组织。用于骨再生的材料必须通过在休息状态和身体运动期间维持机械载荷来提供机械稳定性。骨是由细胞（成骨细胞和破骨细胞）作用不断重塑的动态组织。这种活性通常足以治愈骨损伤；然而，在几种情况下，当骨受到致命损伤时，自我更新是困难的，如果不是不可能的，则需要医疗治疗。生物材料的移植是克服严重损伤的常见手术程序之一。在这项研究中，我们利用了使用不同来源的脱矿骨粉（DBP）与结冷胶（GG）结合形成 GG-DBP 水凝胶支架的效果，目的是再生骨组织。DBP 从两种 domesticus（Yeonsan Ogye，一种来自韩国的传统而罕见的黑鸡，和 Cornish cross，最常用于工业肉类生产的常见品种）和 Pekin 鸭的股骨中提取。已经使用大鼠骨髓间充质干细胞（rBMSCs）进行了 和 研究，证实了 GG-DBP 支架上种子细胞的骨特异性基因表达、分化能力和明显上调的细胞适宜性。与从其他研究来源获得的 DBP 相比，含有源自 Yeonsan Ogye（YO）骨的 DBP 的支架显示出更高的细胞增殖和成骨分化水平。这些结果与 YO DBP 中黑色素的荧光研究量较高有关，与 Cornish cross 和 Pekin duck 相比，YO DBP 中黑色素的荧光研究量较高。总体而言，这项研究清楚地表明，YO DBP 可作为骨组织再生的有前途的材料。
