Komaki Hisayuki, Tamura Tomohiko
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4291-4297. doi: 10.1099/ijsem.0.004287.
We investigated the taxonomic relationships among subsp. , subsp. , and . The 16S rRNA gene sequence similarity between subsp. and subsp. was 97.7 %, whereas subsp. , and showed 100 % nucleotide sequence identity. In addition, subsp. diastaticus and formed a single clade in the phylogenetic tree. Digital DNA-DNA relatedness between subsp. and subsp. was only 22.8%, indicative of different species. In comparison, DNA-DNA relatedness values for subsp. , and ranged from 95.8 to 97.2 %, suggesting the three taxa belong to the same genomospecies. Previously reported phenotypic data also supported synonymy. Therefore, we propose that subsp. should be classified as an independent species, sp. nov. The type strain is NBRC 13412 (=ATCC 3315=CBS 713.72=DSM 40496=ISP 5496=JCM 4745=NBRC 3714=NRRL B-1241=RIA 1373). Our data also suggests that and should be reclassified as later heterotypic synonyms of .
我们研究了亚种、亚种和之间的分类关系。亚种和亚种之间的16S rRNA基因序列相似性为97.7%,而亚种、和显示出100%的核苷酸序列同一性。此外,解淀粉亚种和在系统发育树中形成了一个单一的分支。亚种和亚种之间的数字DNA-DNA相关性仅为22.8%,表明它们是不同的物种。相比之下,亚种、和的DNA-DNA相关性值在95.8%至97.2%之间,表明这三个分类单元属于同一个基因组种。先前报道的表型数据也支持同义关系。因此,我们建议将亚种分类为一个独立的物种,新种。模式菌株是NBRC 13412(=ATCC 3315=CBS 713.72=DSM 40496=ISP 5496=JCM 4745=NBRC 3714=NRRL B-1241=RIA 1373)。我们的数据还表明,和应重新分类为的晚出异名同义词。