Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, PR China.
Nanhu College, Jiaxing University, Jiaxing 314001, PR China.
Bioresour Technol. 2020 Oct;314:123766. doi: 10.1016/j.biortech.2020.123766. Epub 2020 Jul 2.
Endophytic bacteria were isolated from Chlorella vulgaris and co-cultured with its host microalgae to determine whether this symbiotic system is suitable for purifying biogas and biogas slurry. Results showed that endophytic bacteria S395-1 and S395-2 belonged to different genera. Both strains promoted microalgae growth while improving photosynthetic performance, carbonic anhydrase activity, nutrient removal efficiency, and CO fixation. The optimal bacteria (S395-2)-to-microalgae ratio and co-culture duration were 10:1 and 7 days. Under this condition, the growth rate and carbonic anhydrase activity were 0.196 ± 0.06 d and 31.24 ± 0.28 EU/cell, respectively. The symbiotic system had removal efficiencies of 88.29 ± 5.03%, 88.31 ± 4.29%, 88.21 ± 4.51%, and 68.13 ± 1.69% for chemical oxygen demand, nitrogen, phosphorus, and CO, respectively. These results will provide a framework for constructing a microalgal-bacterial consortium that can improve wastewater treatment and enhance biogas quality.
从普通小球藻中分离出内生细菌，并与宿主微藻共培养，以确定这种共生系统是否适合净化沼气和沼液。结果表明，内生细菌 S395-1 和 S395-2 属于不同属。这两株菌均能促进微藻生长，提高光合性能、碳酸酐酶活性、养分去除效率和 CO 固定。最佳细菌（S395-2）与微藻的比例和共培养时间分别为 10:1 和 7 天。在此条件下，生长速率和碳酸酐酶活性分别为 0.196±0.06 d 和 31.24±0.28 EU/细胞。共生系统对化学需氧量、氮、磷和 CO 的去除率分别为 88.29±5.03%、88.31±4.29%、88.21±4.51%和 68.13±1.69%。这些结果将为构建可提高废水处理效果和增强沼气质量的微藻-细菌共生体提供框架。
