National Pingtung University of Science and Technology, Department of Food Science, Pingtung 91201, Taiwan.
National Pingtung University of Science and Technology, Department of Veterinary Medicine, Pingtung 91201, Taiwan.
Food Chem. 2020 Dec 1;332:127389. doi: 10.1016/j.foodchem.2020.127389. Epub 2020 Jun 24.
Food allergens that cause anaphylactic reactions have become an important health problem worldwide. Among them, shrimp is a popular seafood in many cuisines. The best way to avoid allergic reactions is to mitigate the intake of food allergens. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of shrimp DNA. Using LAMP primers, the identification of shrimp DNA by the LAMP assay was specific and rapid (within 30 min). It exhibited no cross-reaction with the DNA of other Crustacea, including crabs and lobster, and at least 0.01% shrimp DNA existed in the test sample. Additionally, the sensitivity of LAMP for detecting shrimp DNA was 100-fold greater than that of conventional PCR. LAMP for the detection of shrimp DNA was reproducible regardless of whether the genomic DNA was extracted from boiled, steamed or roasted shrimp samples. In summary, the LAMP assay established herein not only could be potentially used for diagnosing shrimp DNA but could also be applicable for identifying shrimp allergens in commercial food products in marketplaces.
食物过敏原会引起过敏反应，已成为全球范围内的一个重要健康问题。在这些过敏原中，虾是许多菜肴中很受欢迎的一种海鲜。避免过敏反应的最佳方法是减少食物过敏原的摄入。在这项研究中，开发了一种环介导等温扩增（LAMP）检测方法来检测虾的 DNA。使用 LAMP 引物，LAMP 检测法对虾 DNA 的鉴定具有特异性和快速性（30 分钟内）。它与其他甲壳类动物（包括螃蟹和龙虾）的 DNA 没有交叉反应，并且在测试样品中至少存在 0.01%的虾 DNA。此外，LAMP 检测虾 DNA 的灵敏度比常规 PCR 高 100 倍。无论从煮沸、蒸煮还是烤制的虾样品中提取基因组 DNA，LAMP 检测虾 DNA 的重复性都很好。综上所述，本文建立的 LAMP 检测方法不仅可用于诊断虾 DNA，还可用于鉴定市场上商业食品中的虾过敏原。
