Sheu Shyang-Chwen, Wu Yi-Cheng, Lien Yi-Yang, Lee Meng-Shiou
National Pingtung University of Science and Technology, Department of Food Science, Pingtung, 91201, Taiwan.
National Pingtung University of Science and Technology, Department of Veterinary Medicine, Pingtung, 91201, Taiwan.
Saudi J Biol Sci. 2021 Oct;28(10):5931-5936. doi: 10.1016/j.sjbs.2021.06.057. Epub 2021 Jun 25.
Turmeric () is a rhizomatous plant of the ginger family that is usually dried and ground into powder for use as a seasoning. Because turmeric has become increasingly popular in the functional food market, adulteration of . by other turmeric species is becoming an increasingly significant problem. In this study, loop-mediated isothermal amplification (LAMP) was developed for the detection of DNA for turmeric authentication. ITS2-26S rDNA was used for the LAMP primer designation. The results demonstrated that the specific primers exhibited high specificity, authenticated DNA within 30 min at 65 °C isothermally and had no cross-reaction with other adulterants. LAMP was sensitive to 0.1 ng of turmeric DNA, and only 0.01% of turmeric powder in the sample was required for DNA amplification. The sensitivity of LAMP was 10-fold higher than that of PCR (0.1%) from a previous report. Moreover, all the collected commercial turmeric products were positively detected by LAMP and RtF-LAMP (real-time fluorescence LAMP). The developed LAMP assay not only had higher specificity and rapidity than that of other methods but could also be applied to authenticate turmeric to prevent adulteration in food products.
姜黄是姜科的一种根茎植物，通常经过干燥并研磨成粉末用作调味料。由于姜黄在功能性食品市场越来越受欢迎，姜黄被其他姜黄品种掺假正成为一个日益严重的问题。在本研究中，开发了环介导等温扩增技术（LAMP）用于检测姜黄DNA以进行姜黄鉴定。ITS2 - 26S rDNA用于LAMP引物设计。结果表明，特异性引物表现出高特异性，在65℃等温条件下30分钟内可鉴定姜黄DNA，且与其他掺假物无交叉反应。LAMP对0.1 ng姜黄DNA敏感，样品中仅需0.01%的姜黄粉末即可进行DNA扩增。LAMP的灵敏度比之前报道的PCR（0.1%）高10倍。此外，所有收集的市售姜黄产品均被LAMP和实时荧光LAMP（RtF - LAMP）阳性检测。所开发的LAMP检测方法不仅比其他方法具有更高的特异性和快速性，还可用于鉴定姜黄以防止食品掺假。
