Kamel Ayman H, Amr Abd El-Galil E, Galal Hoda R, Elsayed Elsayed A, Al-Sayady Ahmed I
Department of Chemistry, Faculty of Science, Ain Shams University, 11566 Cairo, Egypt.
Pharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Polymers (Basel). 2020 Jul 7;12(7):1506. doi: 10.3390/polym12071506.
Novel potentiometric devices "ion-selective electrodes (ISEs)" were designed and characterized for the detection of 17β-estradiol (EST) hormone. The selective membranes were based on the use of man-tailored biomimics (i.e., molecularly imprinted polymers (MIPs)) as recognition ionophores. The synthesized MIPs include a functional monomer (methacrylic acid (MAA)) and a cross-linker (ethylene glycol dimethacrylic acid (EGDMA)) in their preparation. Changes in the membrane potential induced by the dissociated 17β-estradiol were investigated in 50 mM CO/HCO buffer solution at pH 10.5. The ion-selective electrodes (ISEs) exhibited fast response and good sensitivity towards 17β-estradiol with a limit of detection 1.5 µM over a linear range starts from 2.5 µM with an anionic response of 61.2 ± 1.2 mV/decade. The selectivity pattern of the proposed ISEs was also evaluated and revealed an enhanced selectivity towards EST over several phenolic compounds. Advantages revealed by the presented sensor (i.e., wide range of assay, enhanced accuracy and precision, low limit of detection, good selectivity, long-term potential stability, rapid response and long life-span and absence of any sample pretreatment steps) suggest its use in routine quality control/quality assurance tests. They were successfully applied to estradiol determination in biological fluids and in different pharmaceutical preparations collected from the local market.
设计并表征了新型电位测定装置“离子选择性电极（ISEs）”，用于检测17β-雌二醇（EST）激素。选择性膜基于使用人工定制的生物模拟物（即分子印迹聚合物（MIPs））作为识别离子载体。合成的MIPs在制备过程中包含功能单体（甲基丙烯酸（MAA））和交联剂（乙二醇二甲基丙烯酸酯（EGDMA））。在pH 10.5的50 mM CO/HCO缓冲溶液中研究了离解的17β-雌二醇引起的膜电位变化。离子选择性电极（ISEs）对17β-雌二醇表现出快速响应和良好的灵敏度，检测限为1.5 µM，线性范围从2.5 µM开始，阴离子响应为61.2 ± 1.2 mV/十倍浓度变化。还评估了所提出的ISEs的选择性模式，结果表明其对EST的选择性高于几种酚类化合物。所展示的传感器具有的优势（即检测范围广、准确性和精密度提高、检测限低、选择性好、长期电位稳定性好、响应快速、寿命长且无需任何样品预处理步骤）表明其可用于常规质量控制/质量保证测试。它们已成功应用于生物流体和从当地市场收集的不同药物制剂中雌二醇的测定。
