Zhang Dingdong, Yan Yanan, Tian Hongyan, Jiang Guangzhen, Li Xiangfei, Liu Wenbin
Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Fish Physiol Biochem. 2018 Feb;44(1):163-173. doi: 10.1007/s10695-017-0421-9. Epub 2017 Sep 10.
Here, we aimed to investigate whether resveratrol (RSV) can ameliorate high-fat diet (HFD)-induced metabolic disorder in fish. Blunt snout bream (Megalobrama amblycephala) with average weight 27.99 ± 0.56 g were fed a normal fat diet (NFD, 5% fat, w/w), a HFD (11% fat), or a HFD supplemented with 0.04, 0.36, or 1.08% RSV for 10 weeks. As expected, fish fed a HFD developed hepatic steatosis, as shown by elevated hepatic and plasma triglycerides, raised whole body fat, intraperitoneal fat ratio and hepatosomatic index, and increased plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST). RSV supplementation lessened increases in body mass, whole body fat, and intraperitoneal fat, and alleviated development of hepatic steatosis, elevations of plasma triglyceride and glucose, and abnormalities of ALT and AST in HFD-fed fish. RSV supplementation increased SIRT1 messenger RNA (mRNA) expression and consequently hepatic mRNA expression of adipose triglyceride lipase (ATGL), carnitine palmitoyltransferase (CPT1a), and microsomal triglyceride transfer protein (MTTP), implying upregulation of lipolysis, β-oxidation, and lipid transport, respectively, in the liver. Conversely, hepatic lipoprotein lipase (LPL), sterol regulatory element-binding protein 1 (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), and ATP citrate lyase (ACLY) mRNA expression were decreased, implying suppression of fatty acid uptake, lipogenesis, and fatty acid synthesis. Additionally, RSV downregulated glucokinase (GCK) and sodium-dependent glucose cotransporter 1 (SGLT1) and upregulated glucose transporter 2 (GLUT2) mRNA expression, thus restoring normal glucose fluxes. Thus, RSV improves lipid and glucose metabolisms in blunt snout bream, which are potentially mediated by activation of SIRT1.
在此，我们旨在研究白藜芦醇（RSV）是否能改善高脂饮食（HFD）诱导的鱼类代谢紊乱。将平均体重为27.99±0.56克的团头鲂投喂正常脂肪饮食（NFD，5%脂肪，w/w）、高脂饮食（11%脂肪）或添加0.04%、0.36%或1.08%RSV的高脂饮食，持续10周。正如预期的那样，投喂高脂饮食的鱼出现了肝脏脂肪变性，表现为肝脏和血浆甘油三酯升高、全身脂肪增加、腹膜内脂肪比例和肝体指数升高，以及血浆丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）升高。补充RSV减少了高脂饮食喂养的鱼的体重、全身脂肪和腹膜内脂肪的增加，并减轻了肝脏脂肪变性的发展、血浆甘油三酯和葡萄糖的升高以及ALT和AST的异常。补充RSV增加了沉默信息调节因子1（SIRT1）信使核糖核酸（mRNA）的表达，从而增加了肝脏中脂肪甘油三酯脂肪酶（ATGL）、肉碱棕榈酰转移酶（CPT1a）和微粒体甘油三酯转移蛋白（MTTP）的mRNA表达，这分别意味着肝脏中脂解、β-氧化和脂质转运的上调。相反，肝脏脂蛋白脂肪酶（LPL）、固醇调节元件结合蛋白1（SREBP-1c）、过氧化物酶体增殖物激活受体γ（PPARγ）和ATP柠檬酸裂解酶（ACLY）的mRNA表达降低，这意味着脂肪酸摄取、脂肪生成和脂肪酸合成受到抑制。此外，RSV下调了葡萄糖激酶（GCK）和钠依赖性葡萄糖共转运蛋白1（SGLT1），并上调了葡萄糖转运蛋白2（GLUT2）的mRNA表达，从而恢复了正常的葡萄糖通量。因此，RSV改善了团头鲂的脂质和葡萄糖代谢，这可能是由SIRT1的激活介导的。
