Baek Su Cheol, Lee Bum Soo, Yi Sang Ah, Yu Jae Sik, Lee Jaecheol, Ko Yoon-Joo, Pang Changhyun, Kim Ki Hyun
School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea.
Laboratory of Nuclear Magnetic Resonance, National Center for Inter-University Research Facilities (NCIRF), Seoul National University, Gwanak-gu, Seoul 08826, Korea.
Plants (Basel). 2020 Jul 7;9(7):858. doi: 10.3390/plants9070858.
L. (Compositae; safflower or Hong Hua) has been used in Korean traditional medicine for maintaining the homeostasis of body circulation. Phytochemical investigation was performed on the florets of by liquid chromatography-mass spectrometry (LC/MS), which afforded two dihydrophaseic acid glucosides ( and ). Isolated compounds were structurally confirmed using a combination of spectroscopic methods including 1D and 2D nuclear magnetic resonance and high-resolution electrospray ionization mass spectroscopy. Their absolute configurations were established by quantum chemical electronic circular dichroism calculations and enzymatic hydrolysis. The anti-adipogenesis activity of the isolated compounds was evaluated using 3T3-L1 preadipocytes. Treatment with the dihydrophaseic acid glucoside () during adipocyte differentiation prevented the accumulation of lipid droplets and reduced the expression of adipogenic genes, and . However, compound did not affect adipogenesis. Our study yielded a dihydrophaseic acid glucoside derived from which has potential advantages for treating obesity.
L.（菊科；红花或红花）在韩国传统医学中用于维持身体循环的稳态。通过液相色谱-质谱联用（LC/MS）对其小花进行了植物化学研究，得到了两种二氢phaseic酸葡萄糖苷（和）。使用包括一维和二维核磁共振以及高分辨率电喷雾电离质谱在内的光谱方法组合对分离出的化合物进行了结构确认。通过量子化学电子圆二色性计算和酶促水解确定了它们的绝对构型。使用3T3-L1前脂肪细胞评估了分离出的化合物的抗脂肪生成活性。在脂肪细胞分化过程中用二氢phaseic酸葡萄糖苷（）处理可防止脂滴积累并降低脂肪生成基因、和的表达。然而，化合物对脂肪生成没有影响。我们的研究得到了一种源自的二氢phaseic酸葡萄糖苷，其在治疗肥胖方面具有潜在优势。
