School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Phytochemistry. 2021 Jul;187:112770. doi: 10.1016/j.phytochem.2021.112770. Epub 2021 Apr 16.
Five previously undescribed polyacetylene glucosides, namely, four C- and one C-acetylenes, together with three known analogues, were isolated from the florets of Carthamus tinctorius L. The structures of these novel compounds were elucidated to be (5R)-5-acetoxy-8,10,12-tetradecatriyne-1-O-β-D-glucopyranoside, (2Z)-decaene-4,6,8-triyne-1-O-β-D-glucopyranoside, (8Z)-1-[(3-O-β-D-glucosyl)-isovaleroyloxy]-8-decaene-4,6-diyne, (8Z)-decaene-1-isovaleroyloxy-4,6-diyne-10-O-β-D-glucopyranoside, and (2E,8E)-decadiene-4,6-diyne-1-O-β-D-glucopyranoside via spectroscopic and chemical methods. All of the isolated compounds were tested for cytotoxicity against cancer cell lines, antibacterial activity, and inhibitory effects on the lipopolysaccharide (LPS)-induced nitric oxide (NO) production. The results showed that (5R)-5-acetoxy-8,10,12-tetradecatriyne-1-O-β-D-glucopyranoside significantly inhibited LPS-induced NO production in RAW264.7 cells in a dose-dependent manner.
从红花的小花中分离得到了五种以前未描述的聚乙炔葡萄糖苷，即四种 C-和一种 C-乙炔，以及三种已知的类似物。这些新化合物的结构被阐明为（5R）-5-乙酰氧基-8,10,12-十四碳三炔-1-O-β-D-吡喃葡萄糖苷、（2Z）-癸烯-4,6,8-三炔-1-O-β-D-吡喃葡萄糖苷、（8Z）-1-[(3-O-β-D-葡萄糖基）-异戊酰氧基]-8-癸烯-4,6-二炔、（8Z）-癸烯-1-异戊酰氧基-4,6-二炔-10-O-β-D-吡喃葡萄糖苷和（2E,8E）-癸二烯-4,6-二炔-1-O-β-D-吡喃葡萄糖苷，通过光谱和化学方法。所有分离得到的化合物均进行了细胞毒性测试，以评估其对癌细胞系的杀伤作用、抗菌活性以及对脂多糖（LPS）诱导的一氧化氮（NO）产生的抑制作用。结果表明，（5R）-5-乙酰氧基-8,10,12-十四碳三炔-1-O-β-D-吡喃葡萄糖苷以剂量依赖的方式显著抑制 RAW264.7 细胞中 LPS 诱导的 NO 产生。
