School of Life Science and Technology, China Pharmaceutical University, Nanjing, People's Republic of China; The State Key Laboratory of Reproductive Medicine, Clinical Center for Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
The State Key Laboratory of Reproductive Medicine, Clinical Center for Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Fertil Steril. 2020 Aug;114(2):331-337. doi: 10.1016/j.fertnstert.2020.04.002. Epub 2020 Jul 7.
To evaluate the effect of coenzyme Q10 (CoQ10) supplementation on oocyte maturation rates and postmeiotic aneuploidy rates during in vitro maturation (IVM) of human oocytes.
Clinical laboratory observation.
Hospital and university laboratories.
PATIENT(S): Forty-five patients aged ≥38 years and 18 patients aged ≤30 years undergoing in vitro fertilization.
INTERVENTION(S): The germinal vesicle-stage oocytes and associated cumulus cells were cultured in IVM media for 24-48 hours with or without 50 μmol/L CoQ10. Oocyte maturation rates were determined based on the presence or absence of the first polar body. Postmeiotic aneuploidies were determined using next-generation sequencing analyses of biopsied polar bodies.
MAIN OUTCOME MEASURE(S): Oocyte maturation rates, postmeiotic oocyte aneuploidy rates, and chromosome aneuploidy frequencies.
RESULT(S): In women aged 38-46 years, 50 μmol/L CoQ10 significantly increased oocyte maturation rates (82.6% vs. 63.0%; P=.035), reduced oocyte aneuploidy rates (36.8% vs. 65.5%; P=.020), and reduced chromosome aneuploidy frequencies (4.1% vs. 7.0%; P=.012. In women aged ≤30 years, we failed to demonstrate an effect of CoQ10 on oocyte maturation rates or postmeiotic aneuploidies.
CONCLUSION(S): CoQ10 supplementation during IVM increased oocyte maturation rates and reduced postmeiotic aneuploidies for older women.
评估辅酶 Q10（CoQ10）补充对人卵母细胞体外成熟（IVM）过程中卵母细胞成熟率和减数后非整倍体率的影响。
临床实验室观察。
医院和大学实验室。
45 名年龄≥38 岁的患者和 18 名年龄≤30 岁的患者进行体外受精。
GV 期卵母细胞及其相关卵丘细胞在 IVM 培养基中培养 24-48 小时，有无 50μmol/L CoQ10。根据有无第一极体来确定卵母细胞成熟率。通过对活检极体进行下一代测序分析来确定减数后卵母细胞的非整倍体率。
卵母细胞成熟率、减数后卵母细胞非整倍体率和染色体非整倍体频率。
在 38-46 岁的女性中，50μmol/L CoQ10 显著提高了卵母细胞成熟率（82.6%对 63.0%；P=.035），降低了卵母细胞非整倍体率（36.8%对 65.5%；P=.020），降低了染色体非整倍体频率（4.1%对 7.0%；P=.012）。在≤30 岁的女性中，我们未能证明 CoQ10 对卵母细胞成熟率或减数后非整倍体有影响。
在 IVM 中补充 CoQ10 可提高高龄女性的卵母细胞成熟率并降低减数后非整倍体率。
