如何在 COVID-19 时代为成人和儿童进行鼻咽拭子检查。
How to perform a nasopharyngeal swab in adults and children in the COVID-19 era.
机构信息
Department of Otorhinolaryngology, Head and Neck Surgery, Tours University Hospital, Tours, France.
Department of pediatric otolaryngology, Head and Neck Surgery, Hôpital Necker-Enfants Malades, AP-HP, Centre - Université de Paris, Paris, France.
出版信息
Eur Ann Otorhinolaryngol Head Neck Dis. 2020 Sep;137(4):325-327. doi: 10.1016/j.anorl.2020.06.001. Epub 2020 Jun 5.
Abstract
The nasopharyngeal swab is currently the main testing method used to diagnose COVID-19. The principle is to collect respiratory cells infected by the virus and to use the RT-PCR (Reverse Transcription - Polymerase Chain Reaction) technique to detect the RNA of the virus. The false negative rate is high, about 30%, which can mainly be explained by an incorrect execution of the technique may increase the false negative rate and decrease the test's sensitivity. The aim of this note is to help healthcare providers to perform this test correctly in adults and children.
摘要
鼻咽拭子是目前诊断 COVID-19 的主要检测方法。其原理是采集感染病毒的呼吸道细胞，通过 RT-PCR（逆转录-聚合酶链反应）技术检测病毒的 RNA。假阴性率较高，约为 30%，这主要可以解释为技术执行不正确可能会增加假阴性率并降低检测的敏感性。本说明的目的是帮助医护人员正确地在成人和儿童中进行此项检测。
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2
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