Food Protection Division, Philippine Center for Postharvest Development and Mechanization, Department of Agriculture, Muñoz, Nueva Ecija, Philippines.
Center for Natural Sciences and Environmental Research (CENSER), De La Salle University, Taft Ave., Manila, Philippines.
Sci Rep. 2020 Jul 9;10(1):11367. doi: 10.1038/s41598-020-68399-z.
The present study focused on the effect of Allium longicuspis extracts (ALE) against anthracnose of mango fruit. In vitro tests (mycelial growth and conidial germination) showed that, ALE concentrated from 0.75 to 2.5 g L completely inhibited the growth of Colletotrichum gloesporioides. Cytoplasmic discharge, mycelial and conidial blasts were clearly observed when applied with ALE. The minimum effective concentration (MEC) of ALE at 0.75 g L can be applied as protective, curative and simultaneous treatment in mango fruit to inhibit the anthracnose infection. Efficacy of garlic extract was relatively superior to synthetic fungicide based on protective, curative and simultaneous treatments. Twenty chemical components were detected in ALE based on GCMS analysis (Table 1). The six major components were the following: oleyl alcohol, methyl ether (42.04%), γ-sitosterol (15.85%), , 24-norursa-3.12-diene (5.62%), 1-octadecanol methyl ether (4.23%), n-pentadecanol (3.95%) and 2-vinyl-4h-1 3-dithiine (3.76%). The findings support the potential use of ALE as an alternative to synthetic fungicide.
本研究聚焦于长茎葱提取物（ALE）对芒果炭疽病的防治作用。体外试验（菌丝生长和分生孢子萌发）表明，浓度为 0.75 至 2.5 g/L 的 ALE 可完全抑制胶孢炭疽菌的生长。当使用 ALE 时，可明显观察到细胞质排放、菌丝和分生孢子破裂。ALE 的最低有效浓度（MEC）为 0.75 g/L，可作为保护剂、治疗剂和同时处理剂应用于芒果果实，以抑制炭疽病感染。大蒜提取物在防治炭疽病方面的功效优于合成杀菌剂。基于 GCMS 分析，在 ALE 中检测到 20 种化学成分（表 1）。其中六个主要成分如下：油醇甲基醚（42.04%）、γ-谷甾醇（15.85%）、24-降乌苏-3,12-二烯（5.62%）、1-十八烷醇甲基醚（4.23%）、正十五烷醇（3.95%）和 2-乙烯基-4h-1,3-二噻嗪（3.76%）。这些发现支持了将 ALE 作为合成杀菌剂替代品的潜在用途。
