Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, India.
Department of Nanoscience and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, India.
Molecules. 2023 Mar 8;28(6):2476. doi: 10.3390/molecules28062476.
The present investigation is focused on exploring the possibilities of identifying biomolecules from the fruiting body of the medicinal mushroom against the mango anthracnose pathogen . The fruiting body (cap and stipe portion) of extracted with ethyl acetate solvent at a maximum inhibitory concentration of 1 percent exhibited the maximum mycelial growth inhibition of with 70.10 percent and 40.77 percent, respectively. Furthermore, subjecting the ethyl acetate extracts from the cap portion of through thin layer chromatography (TLC) revealed the presence of two bands with Rf values of 0.38 and 0.35. The compounds eluted from band 1 recorded with the maximum mycelial growth inhibition of by 53.77 percent followed by band 2 (46.33 percent) using an agar well diffusion test. Similarly, the analysis of ethyl acetate extracts from the cap portion of through Gas Chromatography-Mass spectroscopy (GC-MS) revealed the presence of the organoheterocyclic compound benzothiazole, as expressed in the highest peak area at 22.03 RT with the highest probability percentage (97%). Confirmation of the antifungal nature of benzothiazole was obtained by testing the standard sample of benzothiazole which showed a cent percent of inhibition on mycelial growth of at 50 ppm minimum fungicidal concentration. Furthermore, benzothiazole caused abnormality in the mycelial structures, viz., distortion, shrinkage, clumping of mycelium, conidial malformation, and complete arrestment of conidial germination of as observed through Scanning Electron Microscopy. The research on biomolecular extract of could be a novel and interesting concept for the possibility in suppression of plant pathogenic microbes in the natural field.
本研究旨在探索从药用蘑菇的子实体中鉴定生物分子以对抗芒果炭疽病病原体的可能性。用乙酸乙酯溶剂从子实体（帽和柄部分）中提取，在最大抑制浓度为 1%时，表现出最大的菌丝生长抑制率，分别为 70.10%和 40.77%。此外，通过薄层层析（TLC）对来自 的帽部分的乙酸乙酯提取物进行处理，显示出存在两个带，其 Rf 值分别为 0.38 和 0.35。从带 1 洗脱的化合物记录的最大菌丝生长抑制率为 53.77%，其次是带 2（46.33%），使用琼脂孔扩散试验。同样，通过气相色谱-质谱（GC-MS）分析来自 的帽部分的乙酸乙酯提取物，发现存在有机杂环化合物苯并噻唑，如在最高峰面积为 22.03 RT 的情况下表达，最高概率百分比（97%）。通过测试苯并噻唑的标准样品获得了苯并噻唑的抗真菌性质的确认，该标准样品在 50 ppm 的最低杀菌浓度下显示出对 的菌丝生长的百分之一百的抑制。此外，苯并噻唑导致 的菌丝结构异常，即菌丝扭曲、收缩、菌丝团聚、分生孢子畸形和分生孢子萌发完全停止，通过扫描电子显微镜观察到。对 的生物分子提取物的研究可能是一个新颖而有趣的概念，有可能在自然环境中抑制植物病原微生物。
