Andalib Alireza, Rashed Shadi, Dehbashi Moein, Hajati Jamshid, Noorbakhsh Farshid, Ganjalikhani-Hakemi Mazdak
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, 81746-73461 Iran.
Division of Genetics, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, 81746-73441 Iran.
Indian J Clin Biochem. 2020 Jul;35(3):312-321. doi: 10.1007/s12291-019-00826-z. Epub 2019 Mar 21.
Some microRNAs are usually dysregulated in the cancers and influencing tumor behavior and progression. Hsa-miR-181b-1 and its target are involved in regulating the inflammatory pathways. This study aimed to investigate the expression levels of hsa-mir-181b-1 and in a cohort of breast tumor tissues and normal adjacent tissues to assess their association with breast cancer stages. A total number of 60 breast samples including cancerous and normal adjacent tissue specimens were collected. After pathological study, the expression of hsa-mir-181b-1 and were measured by qRT-PCR method. The hsa-mir-181b-1 expression level was significantly increased in breast tumor tissues compared to the controls. This increase was associated with the disease progression. Conversely, expression level was decreased in tumor samples compared to normal samples, significantly. ROC curve data added other prestigious information of hsa-mir-181b-1 and by defining cancer and healthy tissues with high specificity and sensitivity at a proposed cutoff point. Also, bioinformatic enrichment for the possible targets of mature sequence of "hsa-mir-181b-5p" was performed. Computational analysis showed the five most significant pathways including metabolic, cancer, calcium signaling, PI3K-Akt signaling and focal adhesion pathways which may be influenced by hsa-mir-181b-1. Thus, we suggested hsa-mir-181b-1 and might be involved in the pathogenesis of breast cancer and could be considered as two biomarkers for prediction, prognosis and diagnosis of the stages of the breast cancer.
一些微小RNA在癌症中通常表达失调，并影响肿瘤行为和进展。人源微小RNA-181b-1及其靶标参与调节炎症途径。本研究旨在调查一组乳腺肿瘤组织和邻近正常组织中hsa-mir-181b-1及其靶标的表达水平，以评估它们与乳腺癌分期的关联。总共收集了60份乳腺样本，包括癌组织和邻近正常组织标本。经过病理研究后，采用qRT-PCR方法检测hsa-mir-181b-1及其靶标的表达。与对照组相比，乳腺肿瘤组织中hsa-mir-181b-1的表达水平显著升高。这种升高与疾病进展相关。相反，与正常样本相比，肿瘤样本中其靶标的表达水平显著降低。ROC曲线数据通过在建议的截断点以高特异性和敏感性定义癌组织和健康组织，补充了hsa-mir-181b-1及其靶标的其他重要信息。此外，还对“hsa-mir-181b-5p”成熟序列的可能靶标进行了生物信息学富集分析。计算分析显示了五个最显著的途径，包括代谢、癌症、钙信号、PI3K-Akt信号和粘着斑途径，这些途径可能受hsa-mir-181b-1影响。因此，我们认为hsa-mir-181b-1及其靶标可能参与乳腺癌的发病机制，可被视为乳腺癌分期预测、预后和诊断的两个生物标志物。
