Kinoshita Hiroki, Okabe Hirohisa, Beppu Toru, Chikamoto Akira, Hayashi Hiromitsu, Imai Katsunori, Mima Kosuke, Nakagawa Shigeki, Yokoyama Naomi, Ishiko Takatoshi, Shinriki Satoru, Jono Hirofumi, Ando Yukio, Baba Hideo
Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan ;
Department of Multidisciplinary Treatment for Gastroenterological Cancer, Kumamoto University Hospital, Kumamoto 860-8556, Japan ;
Mol Clin Oncol. 2013 Mar;1(2):309-314. doi: 10.3892/mco.2013.68. Epub 2013 Jan 14.
The cylindromatosis () gene is involved in tumor progression by acting as a negative regulator of nuclear factor-κB (NF-κB). However, the clinical significance of CYLD in patients with hepatocellular carcinoma (HCC) remains unclear. To demonstrate the clinical significance of expression, we analyzed gene expression in 124 paired HCC and non-tumor tissues using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). gene expression was detected in the patients and the cut-off value was determined by the median value of tumor-to-non-tumor (T/N) ratio. qRT-PCR analysis showed that a low expression was associated with a high serum α-fetoprotein (AFP) value. Patients in the low expression group exhibited poorer overall survival compared to those in the high expression group (P=0.0406). Protein expression of CYLD was also investigated in 70 patients with HCC using immunohistochemistry. The findings showed that CYLD protein expression in tumor tissue was associated with gene expression (P=0.031). The findings of the present study suggest that CYLD is clinically associated with tumor development in HCC patients.
圆柱瘤病（）基因作为核因子-κB（NF-κB）的负调节因子参与肿瘤进展。然而，CYLD在肝细胞癌（HCC）患者中的临床意义仍不清楚。为了证明 表达的临床意义，我们使用定量逆转录-聚合酶链反应（qRT-PCR）分析了124对HCC组织和非肿瘤组织中的 基因表达。在患者中检测到 基因表达，并通过肿瘤与非肿瘤（T/N）比值的中位数确定临界值。qRT-PCR分析表明，低 表达与高血清甲胎蛋白（AFP）值相关。低 表达组患者的总生存期比高表达组患者差（P=0.0406）。还使用免疫组织化学对70例HCC患者的CYLD蛋白表达进行了研究。结果表明，肿瘤组织中CYLD蛋白表达与 基因表达相关（P=0.031）。本研究结果表明，CYLD在临床上与HCC患者的肿瘤发展相关。
