佛波醇肉豆蔻酸酯乙酸盐通过一种独立于蛋白激酶C易位的机制诱导HUT 78细胞分泌白细胞介素-2。
Phorbol myristate acetate induces IL-2 secretion by HUT 78 cells by a mechanism independent of protein kinase C translocation.
作者信息
Kelleher D, Pandol S J, Kagnoff M F
机构信息
Department of Medicine, University of California, San Diego, La Jolla 92093.
出版信息
Immunology. 1988 Nov;65(3):351-5.
The protein kinase C (PKC) inhibitor H-7 was used to study the role of PKC in IL-2 secretion. In Jurkat E6.1, a phenotypically non-activated human T-cell line, IL-2 secretion in response to a combination of PMA and the calcium ionophore, ionomycin, or to the mitogen phytohaemagglutinin (PHA) was completely inhibited by H-7. In contrast, HUT-78, a phenotypically activated T-cell line, secreted IL-2 in response to PMA alone, and IL-2 secretion was not inhibited by doses of H-7 that completely blocked PKC activity. We conclude that PMA can stimulate IL-2 secretion independent of the translocation of PKC activity, and suggest that there is an alternative mechanism of action for phorbol esters in activated T cells.
蛋白激酶C（PKC）抑制剂H-7被用于研究PKC在白细胞介素-2（IL-2）分泌中的作用。在Jurkat E6.1（一种表型未激活的人T细胞系）中，H-7完全抑制了细胞对佛波酯（PMA）和钙离子载体离子霉素的联合刺激，或对有丝分裂原植物血凝素（PHA）的刺激所产生的IL-2分泌。相比之下，HUT-78（一种表型激活的T细胞系）仅对PMA就能分泌IL-2，并且其IL-2分泌不受能完全阻断PKC活性的H-7剂量的抑制。我们得出结论，PMA可独立于PKC活性转位来刺激IL-2分泌，并提示佛波酯在激活的T细胞中有另一种作用机制。
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