Long A, O'Connell M, Liskamp R M, Kelleher D
Department of Clinical Medicine, Trinity College Dublin, Ireland.
Immunology. 1993 Dec;80(4):654-7.
Using the T-cell lymphoma line HuT 78, and a clone derived from HuT 78, designated K-4, the role of protein kinase C (PKC) isozymes in the expression of a variety of T-cell surface antigens was investigated. HuT 78 expresses PKC isozymes alpha and beta while K-4 expresses only PKC alpha. Flow cytometric analysis revealed that incubation of HuT 78 cells with phorbol 12-myristate 13-acetate (PMA) results in significant down-regulation of surface expression of CD3. While K-4 cells expressed reduced amounts of CD3, a similar reduction in CD3 expression was not observed when these cells were stimulated with PMA. The regulation of expression of CD11a (LFA-1), CD44, CD45RA and CD45RO and of the class II molecules DR and DP in response to PMA, was similar in both cell lines.
利用T细胞淋巴瘤细胞系HuT 78及其衍生克隆K-4，研究了蛋白激酶C（PKC）同工酶在多种T细胞表面抗原表达中的作用。HuT 78表达PKC同工酶α和β，而K-4仅表达PKCα。流式细胞术分析显示，用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）孵育HuT 78细胞会导致CD3表面表达显著下调。虽然K-4细胞表达的CD3量减少，但用PMA刺激这些细胞时未观察到类似的CD3表达降低。在这两种细胞系中，PMA对CD11a（淋巴细胞功能相关抗原1，LFA-1）、CD44、CD45RA和CD45RO以及II类分子DR和DP表达的调节相似。
