Liu Hao, Zhang Beng, Du Jia, Feng Pei-Pei, Ruan Chen, Zhang Wei-Bo, Zhu Zhong-Hua, Zhou Chi, Li Xin-Wei
Zhejiang Academy of TCM, Hangzhou 310012, China; Department of Acupuncture-Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou 310012.
Department of Acupuncture-Moxibustion, First Affiliated Hospital of Heilongjiang University of CM.
Zhongguo Zhen Jiu. 2020 Jul 12;40(7):757-63. doi: 10.13703/j.0255-2930.20190513-k0003.
To observe the effect of acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) on NLRP3 inflammatory corpuscle in rats with intracerebral hemorrhage (ICH), and to explore the action mechanism of acupuncture on promoting the recovery of neural function in rats with ICH.
Forty SPF six-week-old male SD rats were randomly divided into a sham operation group, a model group, a non-acupoint group and an acupuncture group, 10 rats in each group. The rats in the model group, non-acupoint group and acupuncture group were intervened with autologous blood injection to prepare ICH model, while the rats in the sham operation group were only intervened with operation but not injection with autologous blood. About 3 hours after the establishment of the model, the rats in the acupuncture group were intervened with acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7), once every 12 hours, for 7 days; the rats in the non-acupoint group were intervened with acupuncture at the non-acupoint [parallel to the "Baihui" (GV 20), 1 cm next to the midline] on the affected side, and other treatment was the same as the acupuncture group. At the end of the intervention, the composite nerve function score of each group was evaluated; the histomorphology of the hemorrhage penumbra was observed by HE staining; the expression of NLRP3 inflammatory corpuscle in the brain was detected by immunohistochemistry; the relative protein expression levels of NLRP3, interleukin-1β (IL-1β) and interleukin-18 (IL-18) in brain were detected by the method of Western blot.
Seven days into intervention, compared with the sham operation group, each item score and total score of composite nerve function in the model group were significantly reduced (<0.01, <0.05). There was edema and karyopyknosis in brain neuron as well as necrocytosis and inflammatory cell infiltration in the model group. Compared with the model group and the non-acupoint group, the total score of composite nerve function and the scores of symmetrical movement of limbs (LS) and proprioception of tentacles (VP) in the acupuncture group were increased (<0.01, <0.05), and the cell necrosis and inflammatory cell infiltration were relieved. Compared with the sham operation group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the model group were increased (<0.01); compared with the model group and the non-acupoint group, NLRP3 inflammatory corpuscle expression and the relative protein expression levels of NLRP3, IL-1β and IL-18 in brain tissue in the acupuncture group were reduced (<0.01).
Acupuncture at "Baihui" (GV 20) through "Qubin" (GB 7) could downregulate the expression of NLRP3, IL-1β and IL-18 in the brain tissue of ICH rats, inhibit the inflammatory response, and promote the recovery of neural function.
观察针刺“百会”（GV 20）透“曲鬓”（GB 7）对脑出血（ICH）大鼠NLRP3炎性小体的影响，探讨针刺促进ICH大鼠神经功能恢复的作用机制。
将40只6周龄SPF级雄性SD大鼠随机分为假手术组、模型组、非穴位组和针刺组，每组10只。模型组、非穴位组和针刺组大鼠采用自体血注射法制备ICH模型，假手术组大鼠仅行手术干预，不注射自体血。造模后约3小时，针刺组大鼠针刺“百会”（GV 20）透“曲鬓”（GB 7），每12小时1次，共7天；非穴位组大鼠针刺患侧非穴位[与“百会”（GV 20）平行，中线旁开1 cm]，其余处理同针刺组。干预结束后，评估各组复合神经功能评分；采用HE染色观察出血半暗带组织形态学；采用免疫组化法检测脑组织中NLRP3炎性小体表达；采用Western blot法检测脑组织中NLRP3、白细胞介素-1β（IL-1β）和白细胞介素-18（IL-18）的相对蛋白表达水平。
干预7天时，与假手术组比较，模型组复合神经功能各项评分及总分均显著降低（<0.01，<0.05）。模型组脑神经元有水肿、核固缩，存在细胞坏死及炎性细胞浸润。与模型组和非穴位组比较，针刺组复合神经功能总分及肢体对称运动（LS）、触须本体感觉（VP）评分升高（<0.01，<0.05），细胞坏死及炎性细胞浸润减轻。与假手术组比较，模型组脑组织中NLRP3炎性小体表达及NLRP3、IL-1β、IL-18相对蛋白表达水平升高（<0.01）；与模型组和非穴位组比较，针刺组脑组织中NLRP3炎性小体表达及NLRP3、IL-1β、IL-18相对蛋白表达水平降低（<0.01）。
针刺“百会”（GV 20）透“曲鬓”（GB 7）可下调ICH大鼠脑组织中NLRP3、IL-1β、IL-18的表达，抑制炎症反应，促进神经功能恢复。
