Wang Kaiping, Parekh Udit, Ting Jonathan K, Yamamoto Natasha A D, Zhu Juan, Costantini Todd, Arias Ana Claudia, Eliceiri Brian P, Ng Tse Nga
Department of Electrical and Computer Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Department of Electrical Engineering and Computer Sciences, University of California Berkeley, 253 Cory Hall, Berkeley, CA, 94720, USA.
Adv Biosyst. 2019 Oct;3(10):e1900106. doi: 10.1002/adbi.201900106. Epub 2019 Aug 21.
Wound healing is a complex process involving diverse changes in multiple cell types where the application of electric fields has been shown to accelerate wound closure. To define the efficacy of therapies based on electric fields, it would be valuable to have a platform to systematically study the effects of electrical stimulation (ES) upon the inflammation phase and the activation of signaling mediators. Here, an in vivo ES model in which flexible electrodes are applied to an animal model for monitoring inflammation in a wound is reported on. Subcutaneous implants of polyvinyl alcohol sponges elicit inflammation response as defined by the infiltration of leukocytes. The wound site is subjected to electric fields using two types of additively fabricated flexible electrode arrays. The sponges are then harvested for flow cytometry analysis to identify changes in the phosphorylation state of intracellular targets. This platform enables studies of molecular mechanisms, as it shows that an application of low-frequency ES ≤0.5 Hz increases phosphorylation of Erk proteins in recruited leukocytes, identifying a signaling pathway that is activated during the healing process.
伤口愈合是一个复杂的过程，涉及多种细胞类型的不同变化，其中电场的应用已被证明可加速伤口闭合。为了确定基于电场的治疗方法的疗效，拥有一个平台来系统地研究电刺激（ES）对炎症阶段和信号介质激活的影响将是很有价值的。在此，报道了一种体内ES模型，其中将柔性电极应用于动物模型以监测伤口中的炎症。聚乙烯醇海绵的皮下植入会引发炎症反应，表现为白细胞浸润。使用两种增材制造的柔性电极阵列对伤口部位施加电场。然后收获海绵进行流式细胞术分析，以确定细胞内靶点磷酸化状态的变化。该平台能够研究分子机制，因为它表明施加≤0.5Hz的低频ES会增加募集的白细胞中Erk蛋白的磷酸化，从而确定了在愈合过程中被激活的信号通路。
