Popov S A, Barsov E V, Bukrinskiĭ M I, Zhdanov V M
Mol Gen Mikrobiol Virusol. 1988 Sep(9):36-9.
A fragment of HTLV-IIIB gag-gene, coding for the first 441 amino acids of the p53 gag-precursor was expressed in the recombinant vaccinia virus, vC5. Two HIV specific proteins were detected by western blot in CV-1 cells infected with vC5. Their relative molecular masses were 50 and 35 Kd, pointing out that the first of the proteins is a full length expression product of the cloned sequence, while the second one is a result of processing or abortive translation. Possibilities of using such a strain as a vaccine or in Western blot conformation test are discussed.
编码p53 gag前体前441个氨基酸的HTLV-IIIB gag基因片段在重组痘苗病毒vC5中表达。用蛋白质印迹法在感染vC5的CV-1细胞中检测到两种HIV特异性蛋白。它们的相对分子质量分别为50和35千道尔顿，表明第一种蛋白是克隆序列的全长表达产物，而第二种蛋白是加工或异常翻译的结果。讨论了使用这种毒株作为疫苗或用于蛋白质印迹构象试验的可能性。
