坏死梭杆菌白细胞毒素对牛外周血白细胞的体外作用

Effects of leukocidin from Fusobacterium necrophorum on bovine peripheral leukocytes in vitro.

作者信息

Kanoe M, Yamamoto T, Kai K, Blobel H

机构信息

Department of Veterinary, Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Jun;268(4):463-9. doi: 10.1016/s0176-6724(88)80124-x.

DOI:10.1016/s0176-6724(88)80124-x

PMID:3264983

Abstract

Partially purified leukocidin from Fusobacterium necrophorum damaged bovine peripheral leukocytes as demonstrated by trypan blue staining. Granulocytes were most and T-lymphocytes least sensitive to the leukocidin. Heating for 30 min at 60 degrees C completely inactivated the leukocidin. The cytotoxicity of the leukocidin could be neutralized by homologous anti-leukocidin. Scanning electron microscopy of the exposed cells revealed an apparent destruction of the cell membranes, loss of the microvilli and smoothing of the cell surfaces.

摘要

用台盼蓝染色法证实，从坏死梭杆菌中部分纯化得到的白细胞毒素可损伤牛外周血白细胞。粒细胞对白细胞毒素最为敏感，而T淋巴细胞最不敏感。在60℃加热30分钟可使白细胞毒素完全失活。白细胞毒素的细胞毒性可被同源抗白细胞毒素中和。对暴露细胞进行扫描电子显微镜观察发现，细胞膜明显破坏，微绒毛丧失，细胞表面变得平滑。