Spectrophon Ltd, Oppenheimer 7, Rehovot, 7670107, Israel.
Department of Chemistry, Materials and Chemical Engineering ''Giulio Natta'', Politecnico di Milano, Via Mancinelli 7, Milano, 20131, Italy.
Anal Biochem. 2020 Sep 1;604:113824. doi: 10.1016/j.ab.2020.113824. Epub 2020 Jul 7.
The margins of several pages of the Aleppo codex have been found to be corroded and contaminated by diffuse maculae. In order to understand the origin of this decay these margins have been analysed by applying EVA (ethylene vinyl acetate plastic embedded with strong cation and anion exchangers and mixed with C and C hydrophobic resins) diskettes for harvesting surface material. The captured compounds have been eluted, digested with trypsin and analysed by nano-HPLC-MS. Three major strains of Aspergillus have been identified, namely Aspergillus fumigatus, Aspergillus pseudoglaucus, Aspergillus amstelodami, together with a lactobacillus strain and human keratins. The novelty of this investigation is that for the first time the EVA technology has been applied to ancient parchments in the absence of mechanical deformation or distortion that could be induced if there had been water exchange between the EVA diskettes and the parchment. These findings should help curators to find suitable restoration protocols for these precious documents belonging to the world Cultural Heritage.
几页阿勒颇抄本的页边已被发现被侵蚀和被弥漫性斑点污染。为了了解这种衰变的起源，已经通过应用 EVA（乙烯-醋酸乙烯酯塑料，嵌入有强阳离子和阴离子交换剂，并与 C 和 C 疏水性树脂混合）磁盘来分析这些页边，以收集表面材料。捕获的化合物已被洗脱，用胰蛋白酶消化，并通过纳升 HPLC-MS 进行分析。已经鉴定出三种主要的曲霉属菌株，即烟曲霉、伪苍白曲霉和阿姆斯特丹曲霉，以及一种乳杆菌菌株和人类角蛋白。这项研究的新颖之处在于，首次将 EVA 技术应用于古代羊皮纸，而不会引起机械变形或扭曲，如果 EVA 磁盘和羊皮纸之间存在水分交换，就可能会发生这种情况。这些发现应该有助于馆长为这些属于世界文化遗产的珍贵文献找到合适的修复方案。
