Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, 200031, China; Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA; Department of Biochemistry, West Virginia University, Morgantown, WV, 26506, USA.
Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA.
Exp Eye Res. 2020 Sep;198:108140. doi: 10.1016/j.exer.2020.108140. Epub 2020 Jul 7.
Eyelid basal cell carcinoma (BCC) is the most common eyelid malignancy. Metabolic reprogramming is critical in tumorigenesis, but the metabolic feature of eyelid BCC remains elusive. In this study, we aim to reveal the metabolic profile in eyelid BCC using targeted metabolomics. Eyelid samples were collected from patients who had removal of BCC and from control patients who underwent blepharoplasty. Multivariate analysis of metabolomics data distinguished the two groups, indicating that eyelid BCC has significantly different metabolome than the healthy tissue. We found 16 increased and 11 decreased metabolites in the BCC tissues. These metabolites were highly enriched in the metabolism of nicotinamide adenine dinucleotide (NAD), glutathione metabolism, polyamine metabolism, and the metabolism of glycine, serine, threonine, arginine and proline. amino acid metabolism. Metabolites from NAD metabolism (Nicotinamide; Nicotinamide riboside; N1-Methylnicotinamide) had the highest sensitivity, specificity, and prediction accuracy in a prediction model for eyelid BCC. In conclusion, eyelid BCC has a signature change of cell metabolome. Metabolites in NAD metabolic pathways could potentially be biomarkers or therapeutic targets for eyelid BCC.
眼睑基底细胞癌（BCC）是最常见的眼睑恶性肿瘤。代谢重编程在肿瘤发生中至关重要，但眼睑 BCC 的代谢特征仍难以捉摸。在这项研究中，我们旨在使用靶向代谢组学揭示眼睑 BCC 的代谢特征。从接受 BCC 切除术的患者和接受眼睑成形术的对照患者中采集眼睑样本。代谢组学数据的多元分析将两组区分开来，表明眼睑 BCC 的代谢组与健康组织有明显差异。我们发现 BCC 组织中有 16 种增加的和 11 种减少的代谢物。这些代谢物在烟酰胺腺嘌呤二核苷酸（NAD）代谢、谷胱甘肽代谢、多胺代谢以及甘氨酸、丝氨酸、苏氨酸、精氨酸和脯氨酸代谢中高度富集。氨基酸代谢。在用于预测眼睑 BCC 的预测模型中，NAD 代谢（烟酰胺；烟酰胺核苷；N1-甲基烟酰胺）中的代谢物具有最高的灵敏度、特异性和预测准确性。总之，眼睑 BCC 具有细胞代谢组的特征性变化。NAD 代谢途径中的代谢物可能是眼睑 BCC 的生物标志物或治疗靶点。
