Institute of Biomedicine, Research Center for Infections and Immunity, University of Turku, 20520 Turku, Finland.
Department of Paediatrics and Adolescent Medicine, Turku University Hospital and University of Turku, 20520 Turku, Finland.
Genes (Basel). 2020 Jul 8;11(7):768. doi: 10.3390/genes11070768.
We aimed to explore the role of TLR4 (rs4986790) polymorphism in the nasopharyngeal (NP) bacterial colonization and its consequent impact on the development of childhood asthma. A semi-quantitative culture of NP swabs was performed on 473 children at 2 months of age and on 213 children at 13 months of age. polymorphism was analyzed for 396 children. Children were followed from birth to the age of 7.5 years and the final outcome was physician-diagnosed asthma. The associations between genotype, bacterial colonization, and asthma were analyzed. Children with TLR4 AG or GG genotype were more often colonized with at 2 months of age ( = 0.009) and at 13 months of age ( = 0.018). Children who were colonized with at 13 months of age had a significantly higher risk of later development of asthma ( = 0.004). or colonization at 2 months of age or genotype Asp299Gly were not associated with the development of childhood asthma. Asp299Gly polymorphism was associated with an increased risk of colonization of and in children. The colonization with at 13 months of age was associated with a higher risk of later development of childhood asthma.
我们旨在探讨 TLR4（rs4986790）多态性在鼻咽部（NP）细菌定植中的作用及其对儿童哮喘发展的影响。对 473 名 2 个月大的儿童和 213 名 13 个月大的儿童进行了 NP 拭子的半定量培养。对 396 名儿童进行了多态性分析。对儿童进行了从出生到 7.5 岁的随访，最终结果是医生诊断的哮喘。分析了基因型、细菌定植和哮喘之间的关联。TLR4 AG 或 GG 基因型的儿童在 2 个月大时更容易定植（=0.009）和 13 个月大时定植（=0.018）。在 13 个月大时定植的儿童以后发展为哮喘的风险显著增加（=0.004）。2 个月大时定植或 基因型 Asp299Gly 与儿童哮喘的发展无关。Asp299Gly 多态性与儿童中定植和定植的风险增加有关。13 个月大时定植与以后发展为儿童哮喘的风险增加有关。
