Institute of Biomedicine, Research Center of Infections and Immunity, University of Turku, 20520 Turku, Finland.
Hospital Pediátrico David Bernardino, Luanda 00000, Angola.
Genes (Basel). 2020 Sep 21;11(9):1099. doi: 10.3390/genes11091099.
Bacterial meningitis (BM) is a severe disease caused by various bacterial pathogens. Toll-like receptors (TLRs) protect humans from invading pathogens. In this study, we determined whether single nucleotide polymorphisms (SNPs) of and are associated with susceptibility to and outcome of BM in Angolan children. Samples were taken from 241 patients and 265 age-matched ethnic controls. The SNPs rs4986790 (896A > G) and rs187084 (-1486T > C) were determined by high-resolution melting analysis (HRMA). The frequency of variant genotypes in was significantly higher in patients with meningitis than controls (odds ratio (OR), 2.5; 95% confidence interval (CI), 1.2-5.4; = 0.021), whereas the frequency of variant genotypes in was significantly lower in patients with meningitis than controls (OR, 0.4; 95% CI, 0.2-0.9; = 0.036). No such differences were found with other causative pathogens, such as and . At the time of discharge, patients with meningitis caused by Gram-negative bacteria who were carriers of variant genotypes had a higher risk of ataxia (OR, 12.91; 95% CI, 1.52-109.80; = 0.019) and other neurological sequelae (OR, 11.85; 95% CI, 1.07-131.49; = 0.044) than those with the wild-type genotype. Our study suggests an association between meningitis and genetic variation between and in Angolan children.
细菌性脑膜炎（BM）是由各种细菌病原体引起的严重疾病。Toll 样受体（TLRs）保护人类免受入侵病原体的侵害。在这项研究中，我们确定了 是否存在单核苷酸多态性（SNPs）与安哥拉儿童 BM 的易感性和结果相关。从 241 名患者和 265 名年龄匹配的种族对照中采集样本。通过高分辨率熔解分析（HRMA）确定了 SNPs rs4986790（896A>G）和 rs187084（-1486T>C）的基因型。与对照组相比，患有 脑膜炎的患者中 的变异基因型频率显著更高（比值比（OR），2.5；95%置信区间（CI），1.2-5.4； = 0.021），而患有 脑膜炎的患者中 的变异基因型频率显著更低（OR，0.4；95%CI，0.2-0.9； = 0.036）。对于其他病原体，如 和 ，并未发现这种差异。在出院时，携带变异 基因型的革兰氏阴性菌引起脑膜炎的患者，共济失调的风险更高（OR，12.91；95%CI，1.52-109.80； = 0.019）和其他神经系统后遗症（OR，11.85；95%CI，1.07-131.49； = 0.044）比野生型 基因型的患者更高。我们的研究表明，在安哥拉儿童中， 脑膜炎与 和 之间的遗传变异之间存在关联。
