Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland.
AGH University of Science and Technology, Faculty of Mechanical Engineering and Robotics, Department of Machine Design and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland.
Int J Biol Macromol. 2020 Nov 15;163:1187-1195. doi: 10.1016/j.ijbiomac.2020.07.045. Epub 2020 Jul 9.
Alkaline phosphatase (ALP), biomineralization promoting enzyme, was immobilized in halloysite (HAL) nanotubes and used as a bioactive component of the chitosan (CH) and chitosan-collagen (C-CH) hydrogel scaffolds for bone regeneration. The influence of HAL-ALP and collagen on the properties of the obtained materials was studied. 30 wt% of HAL-ALP increased significantly the swelling ratio of chitosan-based scaffolds. The presence of both: collagen and HAL-ALP had positive effect on the scaffolds' porosity, which was considerably higher for C-CH. The presence of HAL has improved the mechanical properties of both types of scaffolds, while the addition of 20% of collagen to the chitosan hydrogels have considerably decreased their storage modulus. Biomineralization tests showed that although mineral was formed in both CH and C-CH scaffolds with HAL-ALP, the process was more effective for collagen-containing hydrogels. Biological studies, performed using MG-63 osteoblast-like cell line showed that C-CH scaffolds, especially those after biomineralization, were a better material for cell adhesion and growth. Both types of scaffolds degraded slowly in physiological pH. C-CH scaffolds containing 30% of HAL-ALP have the highest potential as bioactive material for bone regeneration.
碱性磷酸酶（ALP）是一种生物矿化促进酶，被固定在埃洛石（HAL）纳米管中，并用作壳聚糖（CH）和壳聚糖-胶原蛋白（C-CH）水凝胶支架的生物活性成分，用于骨再生。研究了 HAL-ALP 和胶原蛋白对所得材料性能的影响。30wt%的 HAL-ALP 显著提高了壳聚糖基支架的溶胀比。同时存在胶原蛋白和 HAL-ALP 对支架的孔隙率有积极影响，C-CH 的孔隙率明显更高。HAL 的存在改善了两种类型支架的机械性能，而向壳聚糖水凝胶中添加 20%的胶原蛋白则大大降低了其储能模量。矿化试验表明，尽管在含有 HAL-ALP 的 CH 和 C-CH 支架中都形成了矿物质，但对于含有胶原蛋白的水凝胶，该过程更有效。使用 MG-63 成骨样细胞系进行的生物学研究表明，C-CH 支架，特别是经过矿化后的支架，是一种更适合细胞黏附和生长的材料。两种支架在生理 pH 值下都缓慢降解。含有 30%HAL-ALP 的 C-CH 支架作为骨再生的生物活性材料具有最大的潜力。
