Machowska Adrianna, Klara Joanna, Ledwójcik Gabriela, Wójcik Kinga, Dulińska-Litewka Joanna, Karewicz Anna
Department of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, Poland.
Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
Polymers (Basel). 2022 Nov 26;14(23):5151. doi: 10.3390/polym14235151.
A new drug delivery system consisting of clindamycin phosphate entrapped in acid-etched halloysite nanotubes was successfully prepared and characterized. It was then used as an antibacterial component of the multicomponent hydrogel designed as a material for bone regeneration. First, halloysite (HNT) was etched and clindamycin phosphate (CP) was entrapped in both raw and modified nanotubes, resulting in HNT-CP and EHNT-CP systems. They were characterized using SEM, TEM, TGA and FTIR; the entrapment efficiency and release of CP from both systems were also studied. EHNT-CP was then used as an antibacterial component of the two hydrogels composed of alginate, collagen and β-TCP. The hydrogels were prepared using different crosslinking procedures but had the same composition. The morphology, porosity, degradation rate, CP release profile, cytocompatibility, antibacterial activity and ability to induce biomineralization were studied for both materials. The hydrogel obtained by a chemical crosslinking with EDC followed by the physical crosslinking with calcium ions had better properties and was shown to have potential as a bone repair material.
成功制备并表征了一种由包裹在酸蚀埃洛石纳米管中的磷酸克林霉素组成的新型药物递送系统。然后将其用作设计用于骨再生材料的多组分水凝胶的抗菌成分。首先，对埃洛石（HNT）进行蚀刻，并将磷酸克林霉素（CP）包裹在原始和改性纳米管中，得到HNT-CP和EHNT-CP系统。使用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、热重分析（TGA）和傅里叶变换红外光谱（FTIR）对它们进行表征；还研究了CP在这两种系统中的包封效率和释放情况。然后将EHNT-CP用作由藻酸盐、胶原蛋白和β-磷酸三钙组成的两种水凝胶的抗菌成分。这些水凝胶采用不同的交联程序制备，但组成相同。对这两种材料的形态、孔隙率、降解率、CP释放曲线、细胞相容性、抗菌活性和诱导生物矿化的能力进行了研究。通过用1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）进行化学交联，然后用钙离子进行物理交联得到的水凝胶具有更好的性能，并显示出作为骨修复材料的潜力。
