Shenzhen Public Platform for Screening and Application of Marine Microbial Resources, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, PR China.
Shenzhen Public Platform for Screening and Application of Marine Microbial Resources, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, PR China.
Water Res. 2020 Sep 15;183:116020. doi: 10.1016/j.watres.2020.116020. Epub 2020 Jun 21.
Elucidating the interactions between algae and associated microbial communities is critical for understanding the mechanisms that mediate the dynamic of harmful algal blooms (HABs) in marine environment. However, the microbial functional profiles and their biogeochemical potential in HABs process remains elusive, especially during a complete natural HAB cycle. Here, we used pyrosequencing and functional gene array (GeoChip) to investigate microbial community dynamics and metabolic potential during a natural dinoflagellate (Noctiluca scintillans) bloom. The results shown that bacterioplankton exhibited significant temporal heterogeneity over the course of the bloom stages. Microbial succession was co-driven by environmental parameters and biotic interactions. The functional analysis revealed significant variations in microbial metabolism during matter cycling. At bloom onset-stage, metabolic potential associated with iron oxidation and transport was elevated. Carbon fixation and degradation, denitrification, phosphorus acquisition, and sulfur transfer/oxidation were significantly enhanced at the plateau stage. During the decline and terminal stages, oxidative stress, lysis of compounds, and toxin degradation & protease synthesis increased. This work reveal phycosphere microorganisms can enhanced organic C decomposition capacity, altered N assimilation rate and S/P turnover efficiency, and balancing of the Fe budget during HAB process. The ecological linkage analysis has further shown that microbial composition and functional potential were significantly linked to algal blooms occurrence. It suggest that structural variability and functional plasticity of microbial communities influence HAB trajectory.
阐明藻类与其相关微生物群落之间的相互作用对于理解海洋环境中有害藻华（HAB）动态的机制至关重要。然而，在 HAB 过程中，微生物的功能特征及其生物地球化学潜力仍然难以捉摸，尤其是在完整的自然 HAB 周期中。在这里，我们使用焦磷酸测序和功能基因芯片（GeoChip）来研究自然甲藻（夜光藻）爆发过程中的微生物群落动态和代谢潜力。结果表明，在整个爆发阶段，细菌浮游生物表现出显著的时间异质性。微生物演替是由环境参数和生物相互作用共同驱动的。功能分析揭示了物质循环过程中微生物代谢的显著变化。在爆发起始阶段，与铁氧化和运输相关的代谢潜力增加。在平台阶段，碳固定和降解、反硝化、磷获取以及硫转移/氧化显著增强。在衰退和终末阶段，氧化应激、化合物裂解以及毒素降解和蛋白酶合成增加。这项工作揭示了噬藻体微生物可以增强有机 C 分解能力、改变 N 同化率和 S/P 周转率效率，并在 HAB 过程中平衡 Fe 预算。生态关联分析进一步表明，微生物组成和功能潜力与藻华的发生显著相关。这表明微生物群落的结构变异性和功能可塑性影响 HAB 的轨迹。
