Department of Histology-Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Biotech Histochem. 2021 Jul;96(5):331-338. doi: 10.1080/10520295.2020.1788161. Epub 2020 Jul 13.
Diabetes mellitus (DM) is a common metabolic disorder with complications including nephropathy, cardiomyopathy, neuropathy and infertility. We investigated nitric oxide synthase (NOS) expression in uterine tissue of diabetic rats. The rats were assigned randomly to four groups of ten: group C, control; group 1, diabetic for 1 month; group 2, diabetic for 2 months; group 3, diabetic for 3 months. DM was induced by streptozotocin injection. nNOS, iNOS, eNOS expressions and TUNEL staining were assessed in uterine tissue and luteinizing hormone (LH), follicle stimulating hormone (FSH) and estrogen were measured in blood samples. The uterine epithelium, connective tissue of the endometrium, myometrium wall thickness and number of apoptotic cells were decreased in groups 1 - 3 compared to group C. nNOS and eNOS expression was increased in diabetic groups, but iNOS expression was similar to group 1. FSH was increased in group 2, but serum LH and estrogen levels were unchanged among groups. DM damages uterine tissue, and NOS, especially nNOS and eNOS, contributes to this damage.
糖尿病（DM）是一种常见的代谢紊乱疾病，其并发症包括肾病、心肌病、神经病和不育症。我们研究了糖尿病大鼠子宫组织中一氧化氮合酶（NOS）的表达。大鼠被随机分为四组，每组 10 只：C 组，对照组；1 组，糖尿病 1 个月；2 组，糖尿病 2 个月；3 组，糖尿病 3 个月。通过链脲佐菌素注射诱导 DM。在子宫组织中评估 nNOS、iNOS 和 eNOS 的表达以及 TUNEL 染色，并在血液样本中测量黄体生成素（LH）、卵泡刺激素（FSH）和雌激素。与 C 组相比，1 组-3 组的子宫上皮、子宫内膜结缔组织、子宫肌壁厚度和凋亡细胞数量减少。糖尿病组中 nNOS 和 eNOS 的表达增加，但 iNOS 的表达与 1 组相似。2 组中 FSH 增加，但各组间血清 LH 和雌激素水平无变化。DM 损害子宫组织，NOS，特别是 nNOS 和 eNOS，促成了这种损害。
