Nest construction and presence do not alter territorial aggression in male threespine stickleback.

Constructing a nest within a mating territory provides a clear benefit to the resident, particularly by improving the opportunity to mate. It is unclear whether animals who use nests exclusively for reproductive purposes account for either the effort invested or the resulting increase in mating potential when valuing their territories. We sought to explicitly reveal a nest's added subjective resource value through within-group comparison of aggressive behaviour before and after nest construction. An increase in aggression following construction would indicate that the resident perceives greater subjective value in his territory, and thus values the nest. Threespine stickleback, , fish demonstrate stereotypical aggressive behaviours during an easily induced territorial defence. The male's nest is used exclusively for reproductive purposes, avoiding any confound of shelter. Contrary to our hypothesis, neither nest presence, timing of construction, nor nesting outcome was associated with differences in behavioural measures of territorial aggression. Assessed behaviours were robust, repeatable and inter-correlated. We conclude that territorial aggression is neither predictive of nor altered by nesting in threespine stickleback fish. Our results suggest that nests used transiently for a portion of the mating season add negligible subjective resource value to a territory. We additionally demonstrate that examinations of territorial aggression in sticklebacks do not need to control for nest building, improving statistical power by decreasing dropout rates. These results dovetail with recent work in other fish species to suggest that assessment of territorial aggression absent a nest may be practicable for fish in general.