使用高效液相色谱法同时测定生物体液中的甲氟喹和氯喹。
Simultaneous determination of mefloquine and chloroquine in biological fluids using high-performance liquid chromatography.
作者信息
Lamant J M, Kintz P, Tracqui A, Mangin P, Lugnier A A, Chaumont A J
机构信息
Institut de Médecine Légale, Strasbourg.
出版信息
Ann Biol Clin (Paris). 1988;46(9):722-4.
A high-performance liquid chromatography (HPLC) method for the simultaneous determination of mefloquine and chloroquine in biological fluids is described. Methaqualone is used as an internal standard. After a single-step extraction, achieved from alkalinized samples with diethyl ether, a separation is obtained using a mu-Bondapak phenyl column with a mobile phase of acetonitrile and sodium acetate buffer. Detection is done at 305 nm. The lower detection limits are 3 and 10 micrograms/l for mefloquine and chloroquine, respectively. Thus, this method appears to be simple, rapid, very sensitive and applicable in pharmaco-clinical, toxicological and forensic analyses.
本文描述了一种同时测定生物流体中甲氟喹和氯喹的高效液相色谱（HPLC）方法。以安眠酮作为内标。用乙醚从碱化样品中进行一步萃取后，使用μ-Bondapak苯基柱，以乙腈和醋酸钠缓冲液作为流动相进行分离。在305nm处进行检测。甲氟喹和氯喹的最低检测限分别为3微克/升和10微克/升。因此，该方法似乎简单、快速、非常灵敏，适用于药物临床、毒理学和法医分析。
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