Nash Robert J, Bartholomew Barbara, Penkova Yana B, Rotondo Dino, Yamasaka Fernanda, Stafford Graham P, Jenkinson Sarah F, Fleet George W J
PhytoQuest Limited, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, U.K.
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, U.K.
ACS Omega. 2020 Jun 23;5(26):16263-16271. doi: 10.1021/acsomega.0c02092. eCollection 2020 Jul 7.
Cucumbers have been anecdotally claimed to have anti-inflammatory activity for a long time, but the active principle was not identified. idoBR1, (2,3,4,5)-3,4,5-trihydroxypiperidine-2-carboxylic acid, is an iminosugar amino acid isolated from fruits of certain cucumbers, (Cucurbitaceae). It has no chromophore and analytically behaves like an amino acid making detection and identification difficult. It has anti-inflammatory activity reducing lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α) in THP-1 cells and human blood. It showed selective inhibition of human α-l-iduronidase and sialidases from both bacteria () and human THP-1 cells. idoBR1 and cucumber extract reduced the binding of hyaluronic acid (HA) to CD44 in LPS-stimulated THP-1 cells and may function as an anti-inflammatory agent by inhibiting induced sialidase involved in the production of functionally active HA adhesive CD44. Similar to the related iminosugars, idoBR1 is excreted unchanged in urine following consumption. Its importance in the diet should be further evaluated.
长期以来，一直有传闻称黄瓜具有抗炎活性，但尚未确定其活性成分。idoBR1，即(2,3,4,5)-3,4,5-三羟基哌啶-2-羧酸，是从某些黄瓜（葫芦科）果实中分离出的一种亚氨基糖氨基酸。它没有发色团，在分析上表现得像氨基酸，这使得检测和鉴定变得困难。它具有抗炎活性，可降低THP-1细胞和人血液中脂多糖（LPS）诱导的肿瘤坏死因子α（TNF-α）。它对来自细菌（）和人THP-1细胞的人α-L-艾杜糖醛酸酶和唾液酸酶具有选择性抑制作用。idoBR1和黄瓜提取物可降低LPS刺激的THP-1细胞中透明质酸（HA）与CD44的结合，并可能通过抑制参与功能性活性HA黏附性CD44产生的诱导型唾液酸酶而发挥抗炎剂的作用。与相关的亚氨基糖类似，食用后idoBR1会原样从尿液中排出。其在饮食中的重要性应进一步评估。
