The sugar specificities of four lactose-binding lectins were studied through the agglutination of adult and/or umbilical cord human erythrocytes (AHRBC and/or CHRBC). 2. Rana catesbeiana egg lectin specifically agglutinated both intact blood group A-AHRBC and intact blood group A-CHRBC. 3. Rana catesbeiana liver lectin agglutinated intact A-AHRBC much more strongly than intact A-CHRBC. 4. Xenopus laevis skin lectin nonspecifically agglutinated AHRBC and CHRBC. 5. Plecoglossus altivelis egg lectin specifically agglutinated intact B-AHRBC, but weakly agglutinated intact B-CHRBC. 6. Comparative studies of lectin-induced AHRBC or CHRBC agglutination clarified the sugar-binding specificities of these lectins.
摘要
通过成人和/或脐带血人类红细胞(成人血红细胞和/或脐血红细胞)的凝集反应研究了四种乳糖结合凝集素的糖特异性。2. 牛蛙卵凝集素能特异性凝集完整的 A 型成人血红细胞和完整的 A 型脐血红细胞。3. 牛蛙肝凝集素对完整的 A 型成人血红细胞的凝集作用比对完整的 A 型脐血红细胞强得多。4. 非洲爪蟾皮肤凝集素能非特异性凝集成人血红细胞和脐血红细胞。5. 香鱼卵凝集素能特异性凝集完整的 B 型成人血红细胞,但对完整的 B 型脐血红细胞的凝集作用较弱。6. 对凝集素诱导的成人血红细胞或脐血红细胞凝集反应的比较研究阐明了这些凝集素的糖结合特异性。