白细胞介素-1α和白细胞介素-1β放射免疫测定法的开发与应用。
Development and application of radioimmunoassays for interleukin-1 alpha and interleukin-1 beta.
作者信息
Poole S, Bristow A F, Selkirk S, Rafferty B
机构信息
Department of Endocrinology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, U.K.
出版信息
J Immunol Methods. 1989 Jan 17;116(2):259-64. doi: 10.1016/0022-1759(89)90212-3.
Specific and sensitive radioimmunoassay (RIA) systems for human IL-1 alpha and IL-1 beta, using sheep polyclonal antisera, have been developed. The assays, which do not require prior extraction of IL-1, have been used to quantify IL-1 alpha and IL-1 beta in synovial fluid and plasma and to quantify intracellular and extracellular IL-1 alpha and IL-1 beta produced by human monocytes stimulated with endotoxin.
已经开发出使用绵羊多克隆抗血清的针对人白细胞介素-1α和白细胞介素-1β的特异性和灵敏的放射免疫测定（RIA）系统。这些测定方法不需要事先提取白细胞介素-1，已被用于定量滑膜液和血浆中的白细胞介素-1α和白细胞介素-1β，以及定量由内毒素刺激的人单核细胞产生的细胞内和细胞外白细胞介素-1α和白细胞介素-1β。
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