Division of Chemistry and Biological Chemistry School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
Chem Asian J. 2020 Sep 1;15(17):2548-2557. doi: 10.1002/asia.202000566. Epub 2020 Jul 28.
Glycoproteins are produced by the post-translational modification process of proteins and they play an important role in mediating various biological processes. Our understanding towards biochemical functions of individual glycoproteins has been seriously hampered due to the heterogeneous expression of carbohydrate parts in glycoproteins. Despite the advancement in recombinant expression and chromatographic techniques, the isolation of pure glycoforms remains nearly impossible. To obtain homogenous glycoproteins, tremendous efforts hves been spent in developing various ligation and glycosylation techniques. This minireview discusses selected methods for the preparation and ligation of glycopeptides. The importance of the development of new chemical synthesis method for glycoproteins has also been discussed, which would be one of the next directions in this field.
糖蛋白是通过蛋白质的翻译后修饰过程产生的，它们在介导各种生物过程中起着重要作用。由于糖蛋白中碳水化合物部分的异质表达，我们对单个糖蛋白的生化功能的理解受到了严重阻碍。尽管在重组表达和色谱技术方面取得了进展，但分离纯的糖型仍然几乎不可能。为了获得同质的糖蛋白，人们付出了巨大的努力来开发各种连接和糖基化技术。本综述讨论了制备和连接糖肽的选定方法。还讨论了开发用于糖蛋白的新化学合成方法的重要性，这将是该领域的下一个方向之一。
