Bioorganic Chemistry, Gebäude NWI, University of Bayreuth, 95440 Bayreuth, Germany.
Department of Chemistry, Graduate School of Science, Osaka University, 1-1, Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
Curr Opin Chem Biol. 2018 Oct;46:130-137. doi: 10.1016/j.cbpa.2018.07.004. Epub 2018 Aug 23.
Glycoproteins have many biological roles. Due to the heterogeneity of natural glycoproteins in the sugar part resulting in glycoforms the evaluation of the biochemical roles of individual glycans remains difficult to investigate. Since pure glycoforms are still not accessible via recombinant or chromatographic methods, the synthesis of proteins with uniform posttranslational modifications using ligation methods or glycan remodeling are currently the best options for accessing these targets. Recent developments in chemical protein synthesis, the assembly of N-glycans and the use of enzymatic procedures have provided access to many glycoproteins with modifications as well as their analogs.
糖蛋白具有多种生物学功能。由于天然糖蛋白在糖部分的异质性导致糖型，因此评估单个聚糖的生化作用仍然难以研究。由于通过重组或色谱方法仍然无法获得纯糖型，因此使用连接方法或糖基重塑合成具有均匀翻译后修饰的蛋白质是目前获得这些靶标的最佳选择。化学蛋白质合成、N-聚糖的组装和酶促程序的最新发展为许多具有修饰的糖蛋白及其类似物提供了途径。
