Coronel Institute of Occupational Health, Amsterdam University Medical Centers, Location AMC, Amsterdam, The Netherlands.
Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, Laboratory for Analytical Chemistry, University of Zagreb, Zagreb, Croatia.
Biomarkers. 2020 Sep;25(6):490-497. doi: 10.1080/1354750X.2020.1792551. Epub 2020 Jul 12.
Prevalence of skin cancer is rapidly increasing. There is a need for non-invasive biomarkers to assess efficacy of prevention strategies aiming at reduction of exposure to ultraviolet radiation (UVR). Recently, stratum corneum (SC) biomarkers were applied in various inflammatory skin diseases. Here, we explore their suitability as candidate biomarkers for UVR.
Twelve volunteers were exposed to a UVB-dose of 0.72 SED, three times a week, during three weeks. As candidate biomarkers, -isomers of urocanic acid (cUCA) and 25 immunological mediators were measured in the SC.
Eight immunological markers significantly changed from baseline. Of them, IL-1RA/IL-1α and a placental growth factor (PIGF) showed gradual changes during UVR-exposure ( < 0.01 for linear trend). cUCA increased sharply already after the first exposure, however, reached a plateau in the second week.
SC represents a promising, non-invasive alternative to skin biopsy in detecting UVR-induced changes. cUCA is the marker of choice for assessment of single UVR-exposure; however, it is less suitable for cumulative UVR-dose. Immunological markers including IL-1RA/IL-1α and PIGF showed gradual changes, and therefore are convenient for monitoring chronic UVR-exposure. These candidate biomarkers might facilitate assessment of the efficacy of preventive measures in the workplace and general population.
皮肤癌的发病率正在迅速上升。因此，需要寻找非侵入性生物标志物，以评估旨在减少紫外线辐射（UVR）暴露的预防策略的效果。最近，角质层（SC）生物标志物已应用于各种炎症性皮肤病。在这里，我们探索它们作为 UVR 候选生物标志物的适用性。
12 名志愿者每周接受三次 0.72 个 SED 的 UVB 剂量照射，共 3 周。作为候选生物标志物，在 SC 中测量了尿刊酸（cUCA）的异构体和 25 种免疫调节剂。
从基线开始有 8 种免疫标志物发生了显著变化。其中，IL-1RA/IL-1α 和胎盘生长因子（PIGF）在 UVR 暴露期间呈逐渐变化（线性趋势 < 0.01）。cUCA 在第一次照射后急剧增加，但在第二周达到平台期。
SC 是一种很有前途的、非侵入性的替代皮肤活检的方法，可用于检测 UVR 诱导的变化。cUCA 是评估单次 UVR 暴露的首选标志物；然而，它不太适合累积 UVR 剂量。包括 IL-1RA/IL-1α 和 PIGF 在内的免疫标志物逐渐发生变化，因此非常适合监测慢性 UVR 暴露。这些候选生物标志物可能有助于评估预防措施在工作场所和一般人群中的效果。
