Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
Curr Opin Struct Biol. 2020 Dec;65:89-95. doi: 10.1016/j.sbi.2020.06.003. Epub 2020 Jul 10.
Single-molecule fluorescence microscopy techniques have enabled the lifecycle of individual RNA transcripts to be quantitatively measured in living cells. The application of these approaches to monitor mRNA degradation, however, has presented a challenge to unequivocally detect these events due to the inherent loss-of-signal resulting from decay of a transcript. Here, we highlight the recent technological developments that have enabled the spatial and temporal dynamics of mRNA degradation of individual transcripts to be visualized within living cells.
单分子荧光显微镜技术使人们能够在活细胞中定量测量单个 RNA 转录本的生命周期。然而，由于转录本衰变导致信号丢失，这些方法在监测 mRNA 降解时，无法明确地检测到这些事件，这对其应用提出了挑战。在这里，我们重点介绍了最近的技术发展，这些发展使人们能够在活细胞内可视化单个转录本的 mRNA 降解的空间和时间动态。
