mRNA 输出的时空调控：单颗粒 RNA 成像提供了新工具和新见解。
Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.
作者信息
Heinrich Stephanie, Derrer Carina Patrizia, Lari Azra, Weis Karsten, Montpetit Ben
机构信息
Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
Department of Cell Biology, University of Alberta, Edmonton, Canada.
出版信息
Bioessays. 2017 Feb;39(2). doi: 10.1002/bies.201600124. Epub 2017 Jan 4.
Abstract
The transport of messenger RNAs (mRNAs) from the nucleus to cytoplasm is an essential step in the gene expression program of all eukaryotes. Recent technological advances in the areas of RNA-labeling, microscopy, and sequencing are leading to novel insights about mRNA biogenesis and export. This includes quantitative single molecule imaging (SMI) of RNA molecules in live cells, which is providing knowledge of the spatial and temporal dynamics of the export process. As this information becomes available, it leads to new questions, the reinterpretation of previous findings, and revised models of mRNA export. In this review, we will briefly highlight some of these recent findings and discuss how live cell SMI approaches may be used to further our current understanding of mRNA export and gene expression.
摘要
信使核糖核酸（mRNA）从细胞核转运至细胞质是所有真核生物基因表达程序中的关键步骤。近期在RNA标记、显微镜技术及测序领域取得的技术进展，正为mRNA的生物合成与输出带来全新见解。这其中包括对活细胞中RNA分子进行的定量单分子成像（SMI），该技术能够提供有关输出过程的时空动态信息。随着这些信息的获取，引发了新的问题、对先前研究结果的重新解读以及对mRNA输出模型的修正。在本综述中，我们将简要介绍其中一些最新发现，并探讨活细胞SMI方法如何用于深化我们目前对mRNA输出及基因表达的理解。
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本文引用的文献
1
Multicolour localization microscopy by point-spread-function engineering.通过点扩散函数工程实现的多色定位显微镜技术
Nat Photonics. 2016;10:590-594. doi: 10.1038/nphoton.2016.137. Epub 2016 Aug 8.
2
Seeing is believing - multi-scale spatio-temporal imaging towards in vivo cell biology.眼见为实——面向体内细胞生物学的多尺度时空成像。
J Cell Sci. 2017 Jan 1;130(1):23-38. doi: 10.1242/jcs.189001. Epub 2016 Aug 9.
3
Altered RNA processing and export lead to retention of mRNAs near transcription sites and nuclear pore complexes or within the nucleolus.RNA加工和输出的改变导致mRNA滞留在转录位点、核孔复合体附近或核仁内。
Mol Biol Cell. 2016 Sep 1;27(17):2742-56. doi: 10.1091/mbc.E16-04-0244. Epub 2016 Jul 6.
4
Translation dynamics of single mRNAs in live cells and neurons.活细胞和神经元中单 mRNA 的翻译动态。
Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5.
5
Real-time quantification of single RNA translation dynamics in living cells.实时定量分析活细胞中单 RNA 翻译动力学。
Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899. Epub 2016 May 5.
6
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7
Dynamic Encounters of Genes and Transcripts with the Nuclear Pore.基因与核孔的动态相互作用。
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8
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