Heinrich Stephanie, Derrer Carina Patrizia, Lari Azra, Weis Karsten, Montpetit Ben
Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
Department of Cell Biology, University of Alberta, Edmonton, Canada.
Bioessays. 2017 Feb;39(2). doi: 10.1002/bies.201600124. Epub 2017 Jan 4.
The transport of messenger RNAs (mRNAs) from the nucleus to cytoplasm is an essential step in the gene expression program of all eukaryotes. Recent technological advances in the areas of RNA-labeling, microscopy, and sequencing are leading to novel insights about mRNA biogenesis and export. This includes quantitative single molecule imaging (SMI) of RNA molecules in live cells, which is providing knowledge of the spatial and temporal dynamics of the export process. As this information becomes available, it leads to new questions, the reinterpretation of previous findings, and revised models of mRNA export. In this review, we will briefly highlight some of these recent findings and discuss how live cell SMI approaches may be used to further our current understanding of mRNA export and gene expression.
信使核糖核酸（mRNA）从细胞核转运至细胞质是所有真核生物基因表达程序中的关键步骤。近期在RNA标记、显微镜技术及测序领域取得的技术进展，正为mRNA的生物合成与输出带来全新见解。这其中包括对活细胞中RNA分子进行的定量单分子成像（SMI），该技术能够提供有关输出过程的时空动态信息。随着这些信息的获取，引发了新的问题、对先前研究结果的重新解读以及对mRNA输出模型的修正。在本综述中，我们将简要介绍其中一些最新发现，并探讨活细胞SMI方法如何用于深化我们目前对mRNA输出及基因表达的理解。
