Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Food Chem. 2020 Dec 15;333:127468. doi: 10.1016/j.foodchem.2020.127468. Epub 2020 Jul 3.
In this study, a new method for simultaneous determination of cyantraniliprole, chlorantraniliprole, tetrachlorantraniliprole, cyclaniliprole and flubendiamide in edible mushrooms by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) combined with a modified QuEChERS procedure. The samples were extracted using acetonitrile and then cleaned up by primary secondary amine (PSA) and octadecylsilane (C18). The determination of these insecticides was achieved in less than 5 min using an electrospray ionization source in positive mode (ESI+) for cyantraniliprole and chlorantraniliprole, while negative mode (ESI-) for tetrachlorantraniliprole, cyclaniliprole and flubendiamide. The linearities of the calibrations for all target compounds were acceptable (R ≥ 0.9922). The limits of detection and quantification were 0.05-2 μg kg and 5 μg kg, respectively. Acceptable recoveries (73.5-110.2%) were acquired for these insecticides with RSDs less than 12.7%. The results demonstrated that the proposed method was effective and convenient for the determination of these insecticides in edible mushrooms.
本研究建立了一种采用高效液相色谱-串联质谱法（HPLC-MS/MS）结合改良 QuEChERS 前处理方法同时测定食用菌中虫螨腈、氯虫苯甲酰胺、四氯虫酰胺、环虫酰肼和氟苯虫酰胺残留量的分析方法。样品经乙腈提取，经 PSA 和 C18 净化后，在电喷雾离子源正模式（ESI+）下测定虫螨腈和氯虫苯甲酰胺，在负模式（ESI-）下测定四氯虫酰胺、环虫酰肼和氟苯虫酰胺。所有目标化合物的校准曲线线性良好（R≥0.9922）。方法的检出限和定量限分别为 0.05-2μg/kg 和 5μg/kg。该方法对这 5 种农药的回收率为 73.5%-110.2%，相对标准偏差（RSD）均小于 12.7%。结果表明，该方法快速、简便、准确，可用于食用菌中这 5 种农药残留的检测。
