Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625 021, Tamil Nadu, India.
Department of Chemistry, Vivekananda College Tiruvedakam West, Madurai 625 234, Tamil Nadu, India.
Food Chem. 2020 Dec 1;332:127150. doi: 10.1016/j.foodchem.2020.127150. Epub 2020 May 28.
We report an optical biosensor using imine, 5-((anthrcene-9-ylmethylene) amino)-2,3dihydrophthalazine) 1-4-dione (ADD) for direct detection of ascorbic acid (AA) via FRET quenched. The ADD was successfully prepared by using simple ultra - sonication method, which was characterized by various spectroscopic techniques. The fluorescence intensity of ADD probe was drastically quenched in presence of AA, and shown excellent selectivity towards the detection of AA in presence of possible biological active interferences. A wide linear range from 0.25 to 190 µM was achieved towards the detection of AA with a LOD of 10 nM. The occurrence of FRET mechanism is due to intermolecular hydrogen bonding between ADD and AA, which was confirmed by Density Functional Theory calculations. Moreover, the biosensor was successfully applied for the detection of AA in real samples such as fruits and vegetables to demonstrate the practicability. In addition, the developed biosensor could be a simple and economically cheap platform for the detection of AA in food samples.
我们报告了一种使用亚胺，5-((蒽-9-基亚甲基)氨基)-2,3-二氢邻苯二甲嗪-1,4-二酮（ADD）的光学生物传感器，通过 FRET 猝灭可直接检测抗坏血酸（AA）。ADD 是通过使用简单的超声处理方法成功制备的，并用各种光谱技术进行了表征。在 AA 的存在下，ADD 探针的荧光强度急剧猝灭，并在存在可能的生物活性干扰的情况下对 AA 的检测表现出优异的选择性。该传感器对 AA 的检测具有从 0.25 到 190 μM 的宽线性范围，LOD 为 10 nM。FRET 机制的发生是由于 ADD 和 AA 之间的分子间氢键，这通过密度泛函理论计算得到了证实。此外，该生物传感器已成功应用于水果和蔬菜等实际样品中 AA 的检测，以证明其实用性。此外，开发的生物传感器可以成为食品样品中 AA 检测的简单且经济便宜的平台。
