Li Shuji, Su Ke, Liu Xing, Jiang Xiuming, Xiang Guoqiang, He Lijun
School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Zhengzhou, 450001, Henan, People's Republic of China.
J Fluoresc. 2024 Jul 31. doi: 10.1007/s10895-024-03868-6.
Nitrogen-doped carbon dots (N-CDs) were prepared by self-exothermic procedure using grasshopper powder as a single precursor. The prepared N-CDs not only have excellent fluorescence properties, but also can catalyze and enhance the ultra-weak chemiluminescence of NaHCO-HO. The reaction conditions of NaHCO-HO-N-CDs CL were optimized. Under the optimal experimental conditions, when AA was added to the NaHCO-HO-N-CDs CL system, AA had a significant inhibitory effect on the CL intensity of NaHCO-HO-N-CDs. There was a good linear relationship between the calculated lg(I0/I) and the concentration of AA (C), and the calibration curve equation was lg(I/I) = 0.03667 C-0.00708 (µM). The established CL analysis method has a detection limit of 0.12 µM for AA and a linear range of 0-50 µM. The selectivity of CL method was evaluated, and the method was successfully applied to the determination of AA in vegetable and fruit samples. The spiked recoveries were between 88.9% and 118.9%, which indicated that the method was simple, rapid, and sensitive, and had great potential in the determination of AA in foods.
以蝗虫粉为单一前驱体，通过自热法制备了氮掺杂碳点（N-CDs）。所制备的N-CDs不仅具有优异的荧光性能，还能催化并增强NaHCO-H₂O₂的超微弱化学发光。对NaHCO-H₂O₂-N-CDs化学发光的反应条件进行了优化。在最佳实验条件下，向NaHCO-H₂O₂-N-CDs化学发光体系中加入AA时，AA对NaHCO-H₂O₂-N-CDs的化学发光强度有显著抑制作用。计算得到的lg(I₀/I)与AA浓度（C）之间存在良好的线性关系，校准曲线方程为lg(I₀/I) = 0.03667C - 0.00708（µM）。所建立的化学发光分析方法对AA的检测限为0.12 µM，线性范围为0 - 50 µM。对化学发光法的选择性进行了评估，该方法成功应用于蔬菜和水果样品中AA的测定。加标回收率在88.9%至118.9%之间，表明该方法简单、快速、灵敏，在食品中AA的测定方面具有很大潜力。
