The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
Talanta. 2017 Apr 1;165:346-350. doi: 10.1016/j.talanta.2016.12.047. Epub 2016 Dec 21.
In this report, a sensitive fluorescent detection of ascorbic acid was developed with protein-stabilized gold nanoclusters. The fluorescence signal from the gold nanoclusters could be effectively quenched by elemental iodine. This kind of quenching could be inhibited by ascorbic acid through a corresponding reduction process, thus a turn-on response toward ascorbic acid was obtained. The validity and performances of the detection scheme were evaluated. The fluorescence responses were linearly related to ascorbic acid in the concentration range 0.1-10μM, and the method was capable of detecting ascorbic acid down to 22nM. The high performances of the developed scheme greatly simplified the sample preparation step, and the method was successfully applied for real sample analyses.
在本报告中，我们利用蛋白质稳定的金纳米簇发展了一种对抗坏血酸进行灵敏荧光检测的方法。金纳米簇的荧光信号可以被元素碘有效地猝灭。这种猝灭可以通过抗坏血酸的相应还原过程来抑制，从而得到了一种对抗坏血酸的开启响应。我们评估了检测方案的有效性和性能。荧光响应与 0.1-10μM 范围内的抗坏血酸呈线性关系，该方法能够检测到低至 22nM 的抗坏血酸。所开发方案的高性能大大简化了样品制备步骤，该方法成功地应用于实际样品分析。
